[选择性激光熔化制备的植入物对早期骨愈合的影响]
[Influence of implants prepared by selective laser melting on early bone healing].
作者信息
Liu J Y, Chen F, Ge Y J, Wei L, Pan S X, Feng H L
机构信息
Department of Prosthodontics, First Clinical Division, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100034, China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2018 Feb 18;50(1):117-122.
OBJECTIVE
To evaluate the influence of the rough surface of dental implants prepared by selective laser melting (SLM) on early bone healing around titanium implants.
METHODS
A total of sixteen titanium implants were involved in our research, of which eight implants were prepared by SLM (TIXOS Cylindrical, Leader-Novaxa, Milan, Italy; 3.3 mm×10 mm, internal hex) and the other eight were sandblasted, large-grit and acid-etched (SLA) implants (IMPLUS Cylindrical, Leader-Novaxa, Milan, Italy; 3.3 mm×10 mm, internal hex). All of the dental implants were inserted into the healed extraction sockets of the mandible of two adult male Beagle dogs. Half of the dental implants were designed to be healed beneath the mucosa and the other half were intended to be healed transgingivally and were immediately loaded by acrylic resin bridge restoration. Three types of tetracycline fluorescent labels, namely calcein blue, alizarin complexone and calcein, were administered into the veins of the Beagle dogs 2, 4, and 8 weeks after implant placement respectively for fluorescent evaluation of newly formed bone peri-implant. Both Beagle dogs were euthanized 12 weeks after implant insertion and the mandible block specimens containing the titanium implants and surrounding bone and soft tissue of each dog were carefully sectioned and dissected. A total of 16 hard tissue slices were obtained and stained with toluidine blue for microscopic examination and histomorphometric measurements. Histological observation was made for each slice under light microscope and laser scanning confocal microscope (LSCM). Comparison on new bone formation around titanium implants of each group was made and mineral apposition rate (MAR) was calculated for each group.
RESULTS
Dental implants prepared by selective laser melting had achieved satisfying osseointegration to surrounding bone tissue after the healing period of 12 weeks. Newly formed bone tissue was observed creeping on the highly porous surface of the SLM implant and growing into the pores of surface structure. Higher MAR values were shown for SLM implants compared with SLA implants (P<0.01).
CONCLUSION
Dental implants prepared by selective laser melting could promote early bone healing and improve mineral apposition rate.
目的
评估选择性激光熔化（SLM）制备的牙种植体粗糙表面对钛种植体周围早期骨愈合的影响。
方法
本研究共纳入16枚钛种植体，其中8枚由SLM制备（TIXOS圆柱形，Leader-Novaxa，意大利米兰；3.3 mm×10 mm，内六角），另外8枚为喷砂、大颗粒酸蚀（SLA）种植体（IMPLUS圆柱形，Leader-Novaxa，意大利米兰；3.3 mm×10 mm，内六角）。所有牙种植体均植入两只成年雄性比格犬下颌愈合的拔牙窝内。一半的牙种植体设计为在黏膜下愈合，另一半设计为经牙龈愈合，并立即用丙烯酸树脂桥修复体加载。分别在种植体植入后2周、4周和8周，向比格犬静脉注射三种四环素荧光标记物，即钙黄绿素蓝、茜素络合物和钙黄绿素，用于对种植体周围新形成骨进行荧光评估。在种植体植入12周后对两只比格犬实施安乐死，并小心地将包含钛种植体以及每只犬周围骨和软组织的下颌骨块标本进行切片和解剖。共获得16片硬组织切片，并用甲苯胺蓝染色以进行显微镜检查和组织形态计量学测量。在光学显微镜和激光扫描共聚焦显微镜（LSCM）下对每片切片进行组织学观察。对每组钛种植体周围的新骨形成进行比较，并计算每组的矿物质沉积率（MAR）。
结果
经过12周的愈合期后，由选择性激光熔化制备的牙种植体与周围骨组织实现了令人满意的骨结合。观察到新形成的骨组织在SLM种植体的高度多孔表面上爬行并长入表面结构的孔隙中。与SLA种植体相比，SLM种植体显示出更高的MAR值（P<0.01）。
结论
选择性激光熔化制备的牙种植体可促进早期骨愈合并提高矿物质沉积率。
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