1型糖尿病合并视网膜病变的中国患者中肾素-血管紧张素系统基因多态性与高血压的相互作用

Interaction of renin-angiotensin system gene polymorphisms with hypertension in Chinese patients with type 1 diabetes and retinopathy.

作者信息

Qiao Yong-Chao, Pan Yan-Hong, Xu Yan, Zhang Xiao-Xi, Zhao Hai-Lu

机构信息

Department of Immunology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China.

Center for Diabetic Systems Medicine, Guangxi Key Laboratory of Excellence, Guilin Medical University, Guilin, Guangxi, China.

出版信息

Oncotarget. 2018 Jan 9;9(7):7582-7589. doi: 10.18632/oncotarget.24100. eCollection 2018 Jan 26.

DOI:10.18632/oncotarget.24100

PMID:29484134

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5800926/

Abstract

BACKGROUND

The objective of this research was to investigate the interaction of RAS gene polymorphisms in Chinese patients with type 1 diabetes mellitus (T1DM) and diabetic retinopathy (DR).

METHODS

Genomic DNA was extracted from peripheral blood leukocytes and genotyping for the angiotensin converting enzyme (ACE) gene I/D and angiotensinogen (AGT) gene M/T polymorphisms was performed using the polymerase chain reaction method. 311 T1DM patients were recruited for the assessment of ACE and AGT polymorphisms relating to DR.

RESULTS

Compared with the diabetic non-retinopathy (DNR) patients, DR patients had lower proportion of diabetic nephropathy (<0.001) and M allele (=0.013). Intriguingly, the frequency D allele (=0.035) was lower in DR patients with hypertension, as well as DD (=0.003) and DI genotype (=0.012) in DR patients with normal blood pressure after multiple tests with Bonferroni correction, but D allele (=0.025) displayed higher in normotensive patients with T1DM. Logistic regression analyses indicated that no significant relationship existed about the genotype and allele polymorphisms with the progress of DR after adjusting for confounding factors.

CONCLUSIONS

Interaction of hypertension and the RAS gene polymorphisms might have a role in the DR development in Chinese T1DM patients.

摘要

背景

本研究的目的是调查中国1型糖尿病（T1DM）患者和糖尿病视网膜病变（DR）患者中RAS基因多态性的相互作用。

方法

从外周血白细胞中提取基因组DNA，采用聚合酶链反应法对血管紧张素转换酶（ACE）基因I/D和血管紧张素原（AGT）基因M/T多态性进行基因分型。招募311名T1DM患者评估与DR相关的ACE和AGT多态性。

结果

与糖尿病无视网膜病变（DNR）患者相比，DR患者的糖尿病肾病比例较低（<0.001），M等位基因比例较低（=0.013）。有趣的是，经过Bonferroni校正的多次检验后，高血压DR患者的D等位基因频率较低（=0.035），血压正常的DR患者的DD基因型（=0.003）和DI基因型（=0.012）较低，但在血压正常的T1DM患者中D等位基因频率较高（=0.025）。逻辑回归分析表明，在调整混杂因素后，基因型和等位基因多态性与DR进展之间不存在显著关系。