Leistra Abigail N, Mihailovic Mia K, Contreras Lydia M
McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA.
Methods Mol Biol. 2018;1737:129-164. doi: 10.1007/978-1-4939-7634-8_9.
Fluorescence-based tools that measure RNA-RNA and RNA-protein interactions in vivo offer useful experimental approaches to probe the complex and dynamic physiological behavior of bacterial RNAs. Here we document the step-by-step design and application of two fluorescence-based methods for studying the regulatory interactions RNAs perform in vivo: (i) the in vivo RNA Structural Sensing System (iRS) for measuring RNA accessibility and (ii) the trifluorescence complementation (TriFC) assay for measuring RNA-protein interactions.
基于荧光的工具可在体内测量RNA-RNA和RNA-蛋白质相互作用，为探究细菌RNA复杂且动态的生理行为提供了有用的实验方法。在此，我们记录了两种基于荧光的方法用于研究RNA在体内执行的调控相互作用的逐步设计和应用：（i）用于测量RNA可及性的体内RNA结构传感系统（iRS），以及（ii）用于测量RNA-蛋白质相互作用的三荧光互补（TriFC）分析。
