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从通迪海岸的蓝蟹(Portunus pelagicus)中分离、鉴定和表征发光细菌,并在高温下进行毒力研究。

Isolation, identification and characterization of the bioluminescent bacteria isolated from the blue swimmer crab Portunus pelagicus along Thondi Coast and virulence studies at high temperatures.

机构信息

Department of Oceanography & Coastal Area Studies, Alagappa University, Thondi Campus, Thondi 623 409, Tamil Nadu, India.

Department of Oceanography & Coastal Area Studies, Alagappa University, Thondi Campus, Thondi 623 409, Tamil Nadu, India.

出版信息

Microb Pathog. 2018 Apr;117:232-236. doi: 10.1016/j.micpath.2018.02.046. Epub 2018 Feb 24.

Abstract

The current study was conducted to isolate the marine bioluminescent bacteria from the blue swimmer crab Portunus pelagicus along the Thondi Coast. Morphological, biochemical and molecular characterization techniques including 16S rRNA gene sequencing and phylogenetic analyses confirmed that the isolated strain was Vibrio harveyi. Experiments were further carried out at different temperatures and various time intervals and the results revealed a significant effects of high temperature and extended time duration on elimination of V. harveyi. Hence, high temperature treatments could facilitate the suppression of V. harveyi from sea food and thereby, preventing food borne infections during human consumption.

摘要

本研究从沿通迪海岸的蓝蟹(Portunus pelagicus)中分离海洋发光细菌。形态学、生物化学和分子特征技术,包括 16S rRNA 基因测序和系统发育分析,证实分离株为哈维氏弧菌(Vibrio harveyi)。进一步在不同温度和不同时间间隔下进行实验,结果表明高温和延长时间对消除哈维氏弧菌有显著影响。因此,高温处理可以促进抑制海鲜中的哈维氏弧菌,从而防止人类食用时发生食源性感染。

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