从藏牦牛（Bos grunniens）中分离的屎肠球菌 JF85 和粪肠球菌 Y83 的特性及其青贮应用。
Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese.
机构信息
Institute of Ensiling and Processing of Grass, College of Prataculture Science, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Ensiling and Processing of Grass, College of Prataculture Science, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Bioresour Technol. 2018 Jun;257:76-83. doi: 10.1016/j.biortech.2018.02.070. Epub 2018 Feb 17.
Two bacteria strains with cellulolytic potential isolated from Tibetan yak (Bos grunniens) rumen were identified as Enterococcus faecalis (JF85) and Enterococcus faecium (Y83). Isolates grow well within a range of temperature 15 to 55 °C and pH 3.0-7.0, respectively. Two strains were inoculated with or without Lactobacillus plantarum (Lp) to Pennisetum sinese silage for 90 days. All inoculants increased lactic acid content, decreased pH and lignocellulose contents compared with silage without additives (control). The lowest pH, highest lactic acid and largest reduction in lignocellulose contents were observed in JF85+Lp and Y83+Lp silages. Isolates alone or in combination with Lp significantly increased WSC, mono- and disaccharides contents as compared to the control. Combined addition efficiently improved enzymatic hydrolysis of Pennisetum sinese silage, indicated by higher glucose yield and cellulose convertibility. Pennisetum sinese ensiled with combined additives is a suitable storage and pretreatment method prior to sugars production from energy crop.
从藏牦牛（Bos grunniens）瘤胃中分离出两株具有纤维素分解能力的细菌菌株，分别鉴定为粪肠球菌（JF85）和屎肠球菌（Y83）。两种分离物在 15 至 55°C 的温度范围内和 pH 3.0-7.0 范围内生长良好。将两种菌株分别与或不与植物乳杆菌（Lp）接种到皇竹草青贮中 90 天。与无添加剂青贮（对照）相比，所有接种剂均增加了乳酸含量，降低了 pH 值和木质纤维素含量。在 JF85+Lp 和 Y83+Lp 青贮中，观察到 pH 值最低、乳酸含量最高和木质纤维素含量降低最大。与对照相比，分离物单独或与 Lp 组合使用显著增加了 WSC、单糖和二糖含量。与对照相比，组合添加物有效地提高了皇竹草青贮的酶解效率，葡萄糖产量和纤维素转化率更高。与联合添加剂青贮的皇竹草是一种适合的储存和预处理方法，可用于从能源作物中生产糖。
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