Zhou Shihang, Liang Xiaohua, Wang Ni, Shao Linnan, Yu Weijian, Liu Ming
a Dalian Blood Center , Dalian , People's Republic of China.
b Department of Cell Biology , Dalian Medical University , Dalian , People's Republic of China.
Hematology. 2018 Sep;23(8):517-521. doi: 10.1080/10245332.2018.1445580. Epub 2018 Feb 27.
Although recent genome-wide association studies have identified a number of single nucleotide polymorphisms associated with platelet count and mean platelet volume (MPV), it is unclear whether polymorphisms in the human platelet antigens (HPA) genes are associated with platelet count and MPV. The aim of this study was to determine the association of the HPA-2, -3, -5 and -15 polymorphisms with platelet count and MPV.
The HPA were genotyped by 5'-nuclease assay in 139 healthy Chinese Han individuals, while platelet count and MPV from the same samples were measured using an hematology cell analyzer.
The platelet count was significantly lower in the individuals with the HPA-2aa genotype compared to those with HPA-2ab (P = 0.020), and significantly higher in individuals with HPA-5aa and HPA-15aa genotypes compared to those with HPA-5ab (P = 0.045) and HPA-15ab/bb (P = 0.032), respectively. On the other hand, platelet count of individuals with the HPA-3aa and HPA-3ab/bb genotypes did not differ significantly (P = 0.084). The MPV was significantly lower in individuals with HPA-5aa genotype compared to those with HPA-5ab (P = 0.001) but did not differ among the HPA-2, -3 and -15 genotypes. Furthermore, HPA-2, -5 and -15 polymorphisms were identified as independent factors for the platelet count and HPA-5 polymorphism was shown as an independent factor for MPV.
This study demonstrates that HPA-2, -5 and -15 polymorphisms are associated with the platelet count while HPA-5 polymorphism is associated with MPV. This finding will further our understanding of the association of HPA polymorphisms with platelet-related diseases.
尽管近期的全基因组关联研究已经确定了一些与血小板计数和平均血小板体积（MPV）相关的单核苷酸多态性，但尚不清楚人类血小板抗原（HPA）基因中的多态性是否与血小板计数和MPV相关。本研究的目的是确定HPA-2、-3、-5和-15多态性与血小板计数和MPV之间的关联。
采用5'-核酸酶分析法对139名健康中国汉族个体的HPA进行基因分型，同时使用血液学细胞分析仪测量同一样本的血小板计数和MPV。
与HPA-2ab基因型个体相比，HPA-2aa基因型个体的血小板计数显著降低（P = 0.020）；与HPA-5ab基因型个体相比，HPA-5aa基因型个体的血小板计数显著升高（P = 0.045），与HPA-15ab/bb基因型个体相比，HPA-15aa基因型个体的血小板计数显著升高（P = 0.032）。另一方面，HPA-3aa基因型个体与HPA-3ab/bb基因型个体的血小板计数无显著差异（P = 0.084）。与HPA-5ab基因型个体相比，HPA-5aa基因型个体的MPV显著降低（P = 0.001），但在HPA-2、-3和-15基因型之间无差异。此外，HPA-2、-5和-15多态性被确定为血小板计数的独立影响因素，HPA-5多态性被证明是MPV的独立影响因素。
本研究表明，HPA-2、-5和-15多态性与血小板计数相关，而HPA-5多态性与MPV相关。这一发现将加深我们对HPA多态性与血小板相关疾病关联的理解。
