Department of Blood Group, Dalian Blood Center, Dalian, China.
Dalian Sixth People's Hospital Affiliated of Dalian Medical University, Dalian, China.
Transfus Apher Sci. 2021 Feb;60(1):102967. doi: 10.1016/j.transci.2020.102967. Epub 2020 Oct 8.
Host genetic polymorphisms influence the fibrosis progression of chronic hepatitis C (CHC) patients. Previous studies have shown the association of human platelet antigens (HPAs) polymorphisms with CHC. However, little is known regarding the association of HPAs polymorphisms with the fibrosis progression of CHC. The aim of this study was to determine the association of HPA -2, -3, -5 and -15 polymorphisms with the levels of serum fibrosis marks in CHC patients.
The HPA -2, -3, -5 and -15 were genotyped by 5'-nuclease assay in 211 CHC patients, while the serum concentration of hyaluronic acid (HA), collagen IV (CIV), amino-terminal pro-peptide of type III procollagen (PIIINP), and laminin (LN) from the same samples were measured by time resolved fluorescence immunoassay.
The level of serum LN was significantly lower in CHC patients with HPA-15aa genotype compared to those with HPA-15ab/bb (P = 0.032) but did not differ among HPA-2, -3 and -5 genotypes. There were no difference in HA, CIV and PIIINP levels among HPA-2, -3,-5 and -15 genotypes.
This study demonstrates that HPA-15 aa polymorphism is associated lower serum LN in CHC, which suggests that HPA -15 aa may be involved in the fibrosis progression of CHC.
宿主遗传多态性影响慢性丙型肝炎（CHC）患者的纤维化进展。先前的研究表明人类血小板抗原（HPAs）多态性与 CHC 相关。然而，对于 HPAs 多态性与 CHC 纤维化进展的相关性知之甚少。本研究旨在确定 HPA-2、-3、-5 和-15 多态性与 CHC 患者血清纤维化标志物水平的关系。
采用 5'-核酸酶法对 211 例 CHC 患者的 HPA-2、-3、-5 和-15 进行基因分型，同时采用时间分辨荧光免疫分析法测定同一样本中透明质酸（HA）、IV 型胶原（CIV）、III 型前胶原氨基端肽（PIIINP）和层粘连蛋白（LN）的血清浓度。
与 HPA-15ab/bb 基因型相比，HPA-15aa 基因型 CHC 患者的血清 LN 水平显著降低（P = 0.032），但 HPA-2、-3 和-5 基因型之间无差异。HPA-2、-3、-5 和-15 基因型之间的 HA、CIV 和 PIIINP 水平无差异。
本研究表明 HPA-15aa 多态性与 CHC 患者血清 LN 降低有关，提示 HPA-15aa 可能参与 CHC 的纤维化进展。
