Belevich T A, Ilyash L V, Milyutina I A, Logacheva M D, Troitsky A V
Lomonosov Moscow State University, Faculty of Biology, Moscow, 119991, Russia.
Biochemistry (Mosc). 2017 Dec;82(12):1538-1548. doi: 10.1134/S0006297917120136.
The molecular diversity of poorly studied algae of Bolidophyceae class was first estimated by Illumina sequencing of V4 region of 18S rRNA gene in ice, under-ice water and summer water of the subarctic White Sea. We used two clustering thresholds - 93 and 97% - and revealed 31 phylotypes of Bolidophyceae. Triparma pacifica and T. strigata were identified to species level. The association of individual phylotypes to certain biotopes (ice or plankton) and stages of seasonal succession (under ice or summer plankton) has been established. Some phylotypes are found in different biotopes and over a wide temperature range. Due to changing their genetic composition, Bolidophyceae are a constant component of the photoautotrophic plankton and ice communities.
通过对北极白海的冰、冰下水和夏季水中18S rRNA基因V4区域进行Illumina测序，首次估计了研究较少的球石藻纲藻类的分子多样性。我们使用了两个聚类阈值——93%和97%——并揭示了31种球石藻纲的系统发育型。太平洋三盘藻和条纹三盘藻被鉴定到种的水平。已经确定了各个系统发育型与特定生物群落（冰或浮游生物）以及季节性演替阶段（冰下或夏季浮游生物）的关联。一些系统发育型存在于不同的生物群落中，且分布在较宽的温度范围内。由于其遗传组成的变化，球石藻纲是光合自养浮游生物和冰群落的一个恒定组成部分。
