Belevich Tatiana A, Milyutina Irina A, Troitsky Aleksey V
Biological Faculty, Lomonosov Moscow State University, 119234 Moscow, Russia.
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia.
Plants (Basel). 2021 Nov 6;10(11):2394. doi: 10.3390/plants10112394.
This survey is the first to explore the seasonal cycle of microbial eukaryote diversity (<3 µm) using the NGS method and a 10-month sediment trap (2018-2019). The long-term trap was deployed from September to June in the northwestern part of the Kara Sea. A water sample collected before the sediment trap was deployed and also analyzed. The taxonomic composition of microbial eukaryotes in the water sample significantly differed from sediment trap samples, characterized by a high abundance of Ciliophora reads and low abundance of Fungi while trap samples contained an order of magnitude less Ciliophora sequences and high contribution of Fungi. Photosynthetic eukaryotes (PEs) accounting for about 34% of total protists reads were assigned to five major divisions: Chlorophyta, Cryptophyta, Dinoflagellata, Haptophyta, and Ochrophyta. The domination of phototrophic algae was revealed in late autumn. Mamiellophyceae and Trebouxiophyceae were the predominant PEs in mostly all of the studied seasons. was constantly present throughout the September-June period in the PE community. The obtained results determine the seasonal dynamics of picoplankton in order to improve our understanding of their role in polar ecosystems.
这项调查首次使用NGS方法和为期10个月的沉积物捕获器（2018 - 2019年）探索了微生物真核生物（<3 µm）的季节性循环。长期沉积物捕获器于9月至6月部署在喀拉海的西北部。在部署沉积物捕获器之前还采集并分析了一份水样。水样中微生物真核生物的分类组成与沉积物捕获器样本有显著差异，其特征是纤毛虫纲读数丰度高而真菌丰度低，而捕获器样本中纤毛虫纲序列数量少一个数量级且真菌贡献高。占原生生物读数总量约34%的光合真核生物（PEs）被归为五个主要门类：绿藻门、隐藻门、甲藻门、定鞭藻门和褐藻门。在深秋发现了光合藻类的优势地位。在几乎所有研究季节中，扁藻科和小球藻科都是主要的光合真核生物。在9月至6月期间，PE群落中一直存在。所获得的结果确定了微微型浮游生物的季节动态，以增进我们对它们在极地生态系统中作用的理解。
