Brito Dayane R, Carbonero Elaine R, Viana Sthefany R F, Silva Estefânia V, Ruthes Andrea Caroline, Lião Luciano M, Iacomini Marcello
Departamento de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020 Catalão, Brazil.
Departamento de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020 Catalão, Brazil.
Carbohydr Res. 2018 Mar 22;458-459:29-34. doi: 10.1016/j.carres.2018.02.002. Epub 2018 Feb 6.
Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides. In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution. The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 10 g/mol and 28.5 × 10 g/mol, respectively. Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.
金顶侧耳，俗称“金平菇”，具有药用特性，这主要归因于生物活性多糖的存在。在这项研究中，通过连续水提取，随后进行冻融分级，并使用费林溶液沉淀可溶物质，从这种真菌的子实体中分离出两种部分3 - O - 甲基化的半乳聚糖。使用一维和二维核磁共振光谱、单糖组成和甲基化分析进行结构鉴定。这些多糖的特征为线性、部分3 - O - 甲基化的（1→6）连接的α - 吡喃半乳糖聚糖，仅含有半乳糖（Gal）和3 - O - 甲基半乳糖（3 - O - Me - Gal），摩尔比分别为2:1和1:1，摩尔质量分别为37.6×10 g/mol和28.5×10 g/mol。其他侧耳属物种也有类似结构的报道，但3 - O - Me - Gal的含量较低。
