School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Testing Center, Yangzhou University, Wenhui East Rd. 48, Yangzhou 225009, China.
Int J Biol Macromol. 2018 May;111:1293-1303. doi: 10.1016/j.ijbiomac.2018.01.090. Epub 2018 Feb 1.
A novel bioactive polysaccharide, GFP-22, was isolated from the fruit bodies of Grifola frondosa by anion-exchange and gel filtration chromatography. Structure of GFP-22 was investigated using gas chromatography-mass spectrometry (GC-MS), methylation, nuclear magnetic resonance (NMR), high-performance size exclusion chromatography-multi-angle laser light scattering-refractive index detector (HPSEC-MALLS-RI) and atomic force microscopy (AFM) analysis. The backbone of GFP-22 is composed of 1,4-β-d-Glcp, 1,3-β-d-Glcp, 1,6-α-d-Glcp, 1,6-α-d-Galp, 1,4,6-α-d-Manp and 1,3,6-α-d-Manp units. Molecular weight of GFP-22 is 2.72 × 10 Da. GFP-22 has a linear filamentous structure. The administration of GFP-22 could improve or reverse the CTX-induced immunosuppression, significantly enhance the spleen and thymus indices, spleen lymphocyte proliferation and cytokines production in splenocytes. These findings suggest that GFP-22 could be explored as a natural and potential immunomodulatory agent and as an alternative means of lessening chemotherapy-induced immunosuppression.
一种新型的生物活性多糖 GFP-22 从灰树花的子实体中通过阴离子交换和凝胶过滤层析分离得到。使用气相色谱-质谱联用（GC-MS）、甲基化、核磁共振（NMR）、高效体积排除色谱-多角度激光散射-折射率检测器（HPSEC-MALLS-RI）和原子力显微镜（AFM）分析对 GFP-22 的结构进行了研究。GFP-22 的骨架由 1,4-β-d-Glcp、1,3-β-d-Glcp、1,6-α-d-Glcp、1,6-α-d-Galp、1,4,6-α-d-Manp 和 1,3,6-α-d-Manp 单元组成。GFP-22 的分子量为 2.72×104 Da。GFP-22 具有线性丝状结构。GFP-22 的给药可以改善或逆转 CTX 诱导的免疫抑制，显著提高脾和胸腺指数，脾淋巴细胞增殖和脾细胞细胞因子的产生。这些发现表明 GFP-22 可以作为一种天然的、潜在的免疫调节剂，作为减轻化疗诱导的免疫抑制的一种替代方法进行探索。
