Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
Carbohydr Polym. 2018 May 1;187:19-25. doi: 10.1016/j.carbpol.2018.01.047. Epub 2018 Feb 4.
A homogenous polysaccharide (APP), with a molecular weight of 120 kDa, was isolated from the dried aerial parts of Agrimonia pilosa. Gas chromatography (GC) and GC-MS analysis revealed that APP has a backbone of 1,3-linked Glcp and 1,3, 6-linked Glcp, and branched with 1-linked Glcp terminal along the main chain in a relative ratio of 2:1:1. We investigated the response of human osteosarcoma U-2 OS cells to APP treatment. MTT result showed that APP significantly inhibited cell viability in a concentration dependent manner via induction of apoptotic death in U-2 OS cells, as determined by annexin V/propidium iodide (PI) staining. Western blot analysis also indicated that APP CRA increased in Bax/Bcl-2 ratios by up-regulating Bax expression and triggered the release of cytochrome c from mitochondria into the cytoplasm. Moreover, APP supplement induced the activation of caspase-3, and -9, but not caspase-8 in U-2 OS cells. Likewise, APP administration significantly suppressed tumor growth in BALB/C nude mice bearing U-2 OS xenograft tumors. All these results indicate that APP-induced apoptosis is associated with the activation of a caspase-3-mediated mitochondrial pathway.
从龙芽草的干燥地上部分中分离得到一种均一的多糖（APP），其分子量为 120 kDa。气相色谱（GC）和 GC-MS 分析表明，APP 的主链由 1,3 连接的 Glcp 和 1,3,6 连接的 Glcp 组成，并且沿着主链以 1:1:1 的相对比例分支有 1 连接的 Glcp 末端。我们研究了 APP 处理对人骨肉瘤 U-2 OS 细胞的反应。MTT 结果表明，APP 通过诱导 U-2 OS 细胞凋亡，以浓度依赖的方式显著抑制细胞活力，通过 Annexin V/PI（碘化丙啶）染色来确定。Western blot 分析还表明，APP CRA 通过上调 Bax 表达增加 Bax/Bcl-2 比值，并触发细胞色素 c 从线粒体释放到细胞质中。此外，APP 补充剂诱导 U-2 OS 细胞中 caspase-3 和 -9 的激活，但不诱导 caspase-8 的激活。同样，APP 给药显著抑制了携带 U-2 OS 异种移植肿瘤的 BALB/C 裸鼠中的肿瘤生长。所有这些结果表明，APP 诱导的细胞凋亡与 caspase-3 介导的线粒体途径的激活有关。
