Department of Molecular Biology, University Medical Centre Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.
Department of Molecular Biology, University Medical Centre Göttingen, Humboldtallee 23, 37073 Göttingen, Germany; Göttingen Center for Molecular Biosciences, University of Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
Trends Biochem Sci. 2018 Apr;43(4):237-250. doi: 10.1016/j.tibs.2018.02.001. Epub 2018 Feb 24.
RNA helicases are critical regulators at the nexus of multiple pathways of RNA metabolism, and in the complex cellular environment, tight spatial and temporal regulation of their activity is essential. Dedicated protein cofactors play key roles in recruiting helicases to specific substrates and modulating their catalytic activity. Alongside individual RNA helicase cofactors, networks of cofactors containing evolutionarily conserved domains such as the G-patch and MIF4G domains highlight the potential for cross-regulation of different aspects of gene expression. Structural analyses of RNA helicase-cofactor complexes now provide insight into the diverse mechanisms by which cofactors can elicit specific and coordinated regulation of RNA helicase action. Furthermore, post-translational modifications (PTMs) and long non-coding RNA (lncRNA) regulators have recently emerged as novel modes of RNA helicase regulation.
RNA 解旋酶是 RNA 代谢多个途径交汇点的关键调控因子，在复杂的细胞环境中，对其活性进行严格的时空调控至关重要。专门的蛋白质辅助因子在将解旋酶募集到特定底物并调节其催化活性方面发挥着关键作用。除了单个 RNA 解旋酶辅助因子外，含有 G 补丁和 MIF4G 结构域等进化保守结构域的辅助因子网络突出了不同方面的基因表达的交叉调控的可能性。RNA 解旋酶-辅助因子复合物的结构分析现在为辅助因子可以引发 RNA 解旋酶作用的特异性和协调调节的多种机制提供了深入了解。此外，翻译后修饰（PTMs）和长非编码 RNA（lncRNA）调节剂最近已成为 RNA 解旋酶调节的新方式。
