UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia.
Department of Obstetrics and Gynecology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Biosci Rep. 2018 Mar 29;38(2). doi: 10.1042/BSR20171268. Print 2018 Apr 27.
Information on the prevalence and type distribution of human papillomavirus (HPV) among Malaysian women is currently limited. The present study therefore aimed to provide an updated estimate on the prevalence and type distribution of HPV among Malaysian women with and without cervical cancer. Total DNA was isolated from the cervical cell specimens of 185 histopathologically confirmed cervical cancer patients and 209 cancer-free healthy females who were tested negative in a recent Pap test. Viral-specific DNA was subsequently amplified with biotinylated primers and hybridized to HPV type-specific probes via a proprietary "flow-through hybridization" process for determination of HPV genotype. It was demonstrated that 83.2% of the cervical cancer patients and none (0.0%) of the cancer-free females were positive for HPV infection. Among HPV-positive subjects, 14 different viral genotypes were observed, namely HPV16, 18, 31, 33, 35, 45, 52, 53, 58, 66/68, 73, 81, 82, and 84/26. A total of 91.6% of the HPV-positive subjects had single-type HPV infections and the remaining 8.4% were simultaneously infected by two HPV genotypes. The most common HPV infections found were HPV16 (35.7%), HPV18 (26.0%), HPV58 (9.1%), and HPV33 (7.1%) single-type infections, followed by HPV16 + HPV18 co-infections (5.2%). The study has successfully provided an updated estimate on the prevalence and type distribution of HPV among Malaysian women with and without cervical cancer. These findings could contribute valuable information for appraisal of the impact and cost-effectiveness of prophylactic HPV vaccines in the Malaysian population.
马来西亚女性的人乳头瘤病毒（HPV）流行率和型别分布信息目前有限。因此，本研究旨在提供马来西亚宫颈癌患者和无宫颈癌健康女性 HPV 流行率和型别分布的最新估计。从 185 例经组织病理学证实的宫颈癌患者和 209 例最近巴氏试验阴性的无癌健康女性的宫颈细胞标本中提取总 DNA。随后，用生物素标记的引物扩增病毒特异性 DNA，并通过专有“流通过程杂交”过程与 HPV 型特异性探针杂交，以确定 HPV 基因型。结果表明，83.2%的宫颈癌患者和 0.0%的无癌女性 HPV 感染阳性。在 HPV 阳性者中，观察到 14 种不同的病毒基因型，即 HPV16、18、31、33、35、45、52、53、58、66/68、73、81、82 和 84/26。91.6%的 HPV 阳性者为单型 HPV 感染，其余 8.4%为两种 HPV 基因型同时感染。最常见的 HPV 感染是 HPV16（35.7%）、HPV18（26.0%）、HPV58（9.1%）和 HPV33（7.1%）单型感染，其次是 HPV16+HPV18 共感染（5.2%）。该研究成功提供了马来西亚宫颈癌患者和无宫颈癌健康女性 HPV 流行率和型别分布的最新估计。这些发现可为评估预防性 HPV 疫苗在马来西亚人群中的影响和成本效益提供有价值的信息。
