Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Korea.
National Research Laboratory of Molecular Microbiology and Toxicology, Seoul National University, Seoul 08826, Korea.
Mol Cells. 2018 Apr 30;41(4):301-310. doi: 10.14348/molcells.2018.2190. Epub 2018 Feb 27.
LysR-type transcriptional regulators (LTTRs) contain an N-terminal DNA binding domain (DBD) and a C-terminal regulatory domain (RD). Typically, LTTRs function as homotetramers. VV2_1132 was identified in as an LTTR that is a homologue of HypT (also known as YjiE or QseD) in . In this study, we determined the crystal structure of full-length VV2_1132 at a resolution of 2.2 Å, thereby revealing a novel combination of the domains in the tetrameric assembly. Only one DBD dimer in the tetramer can bind to DNA, because the DNA binding motifs of the other DBD dimer are completely buried in the tetrameric assembly. Structural and functional analyses of VV2_1132 suggest that it might not perform the same role as HypT, indicating that further study is required to elucidate the function of this gene in . The unique structure of VV2_1132 extends our knowledge of LTTR function and mechanisms of action.
LysR 型转录调控因子 (LTTRs) 包含一个 N 端 DNA 结合结构域 (DBD) 和一个 C 端调节结构域 (RD)。通常，LTTRs 作为同源四聚体发挥作用。VV2_1132 在 中被鉴定为 HypT（也称为 YjiE 或 QseD）的同源物。在这项研究中，我们确定了全长 VV2_1132 的晶体结构，分辨率为 2.2 Å，从而揭示了四聚体组装中结构域的一种新组合。四聚体中只有一个 DBD 二聚体可以与 DNA 结合，因为另一个 DBD 二聚体的 DNA 结合基序完全埋在四聚体组装中。VV2_1132 的结构和功能分析表明，它可能不起与 HypT 相同的作用，这表明需要进一步研究来阐明该基因在 中的功能。VV2_1132 的独特结构扩展了我们对 LTTR 功能和作用机制的认识。
