Farkas R, Manor Y, Klajman A
Clin Immunol Immunopathol. 1987 Feb;42(2):171-82. doi: 10.1016/0090-1229(87)90004-3.
B suppressor cells (Bs) were generated by stimulation with PHA-P or concanavalin A of B cells from peripheral blood of B-cell chronic lymphocytic leukemia (B-CLL) patients. They suppressed well allogeneic mixed lymphocyte reactions. Long-term colonies of B cells from B-CLL were established for up to 6 weeks in liquid media, with B cells acquiring properties of suppressor cells while being cultured. Two types of cultures were observed: cells either grew in compact multicellular colonies or formed diffuse monolayers. Cells that remained alive in cultures for a number of weeks, but did not multiply, did not develop suppressive characteristics. Bs cells retained their phenotypic markers in cultures. Mitosis and lymphoblasts, but no differentiation into plasma cells, were observed. PHA-P-generated Bs were cultured in long-term colonies, retaining their suppressor properties. The establishment of long-term cultures of B-CLL cells may facilitate a better understanding of the nature and characteristics of normal and leukemic B cells.
通过用PHA-P或刀豆球蛋白A刺激B细胞慢性淋巴细胞白血病（B-CLL）患者外周血中的B细胞，产生了B抑制细胞（Bs）。它们能很好地抑制同种异体混合淋巴细胞反应。在液体培养基中建立了来自B-CLL的B细胞长期集落，长达6周，B细胞在培养过程中获得了抑制细胞的特性。观察到两种培养类型：细胞要么在紧密的多细胞集落中生长，要么形成弥漫性单层。在培养物中存活数周但不增殖的细胞没有发展出抑制特性。Bs细胞在培养物中保留了它们的表型标记。观察到有丝分裂和淋巴母细胞，但没有分化为浆细胞。用PHA-P产生的Bs在长期集落中培养，保留了它们的抑制特性。B-CLL细胞长期培养物的建立可能有助于更好地理解正常和白血病B细胞的性质和特征。
