Laboratory for Clinical Immunology Meir Hospital Kfar-Saba 44281 Israel.
Mediators Inflamm. 1997;6(2):147-53. doi: 10.1080/09629359791857.
Interleukin-6 (IL-6) and soluble interleukin-6 receptor (sIL-6R) were detected in supernatants of cultures of B chronic lymphatic leukaemia (CLL) lymphocytes. Phorbol-12-myristate 13 acetate (PMA) caused a decrease in the levels of IL-6 in 14 out of 16 cultures and an increase in levels of sIL6R in all 15 cases. The effect of pokeweed mitogen (PWM) was variable and not significant. The levels of IL-6 were below the detection limit (60 pg/ml) in sera of 13 CLL patients whereas sIL-6R was detected (13 ng/ml to 97 ng/ml) in the 13 sera. IL6 was not detected in cultures of unstimulated or stimulated with PMA or PWM normal human B cells. Levels of sIL-6R were minimal in cultures of normal B lymphocytes and were increased in PMA stimulated cultures. The results are consistent with the view that B-CLL cells produce spontaneously IL-6 which could act in an autocrine fashion to cause shedding of surface IL-6R and account for the correlation found between serum levels of sIL-6R and B-CLL lymphocyte numbers. The fall in levels of IL-6 in PMA stimulated CLL cultures might express masking or degradation of IL-6 after combination with the receptor.
白细胞介素-6（IL-6）和可溶性白细胞介素-6 受体（sIL-6R）在慢性 B 淋巴细胞白血病（CLL）淋巴细胞培养的上清液中被检测到。佛波醇-12-肉豆蔻酸 13-醋酸酯（PMA）导致 16 个培养物中的 14 个培养物中 IL-6 水平降低，所有 15 个培养物中 sIL6R 水平增加。美洲商陆丝裂原（PWM）的作用是可变的，没有统计学意义。13 例 CLL 患者的血清中 IL-6 水平低于检测限（60pg/ml），而 13 例血清中均检测到 sIL-6R（13ng/ml 至 97ng/ml）。未刺激或用 PMA 或 PWM 刺激的正常人 B 细胞培养物中未检测到 IL6。sIL-6R 的水平在正常 B 淋巴细胞培养物中最低，在 PMA 刺激的培养物中增加。这些结果与以下观点一致，即 B-CLL 细胞自发产生 IL-6，可能以自分泌方式发挥作用，导致表面 IL-6R 的脱落，并解释了在血清 sIL-6R 水平与 B-CLL 淋巴细胞数量之间发现的相关性。PMA 刺激的 CLL 培养物中 IL-6 水平下降可能表示 IL-6 与受体结合后被掩盖或降解。
