Chauhan Nar S, Pandey Rajesh, Mondal Anupam K, Gupta Shashank, Verma Manoj K, Jain Sweta, Ahmed Vasim, Patil Rutuja, Agarwal Dhiraj, Girase Bhushan, Shrivastava Ankita, Mobeen Fauzul, Sharma Vikas, Srivastava Tulika P, Juvekar Sanjay K, Prasher Bhavana, Mukerji Mitali, Dash Debasis
Department of Biochemistry, Maharshi Dayanand University, Rohtak, India.
CSIR Ayurgenomics Unit - TRISUTRA (Translational Research and Innovative Science ThRough Ayurgenomics), CSIR-Institute of Genomics and Integrative Biology, New Delhi, India.
Front Microbiol. 2018 Feb 13;9:118. doi: 10.3389/fmicb.2018.00118. eCollection 2018.
Heterogeneity amidst healthy individuals at genomic level is being widely acknowledged. This, in turn, is modulated by differential response to environmental cues and treatment regimens, necessitating the need for stratified/personalized therapy. We intend to understand the molecular determinants of Ayurvedic way (ancient Indian system of medicine) of endo-phenotyping individuals into distinct constitution types termed " which forms the basis of personalized treatment. In this study, we explored and analyzed the healthy human gut microbiome structure within three predominant groups from a genetically homogenous cohort to discover differentially abundant taxa, using 16S rRNA gene based microbial community profiling. We found Bacteroidetes and Firmicutes as major gut microbial components in varying composition, albeit with similar trend across . Multiple species of the core microbiome showed differential abundance within types, with gender specific signature taxons. Our study reveals that despite overall uniform composition of gut microbial community, healthy individuals belonging to different groups have enrichment of specific bacteria. It highlights the importance of based endo-phenotypes to explain the variability amongst healthy individuals in gut microbial flora that have important consequences for an individual's health, disease and treatment.
基因组水平上健康个体之间的异质性正得到广泛认可。反过来，这又受到对环境线索和治疗方案的不同反应的调节，因此需要分层/个性化治疗。我们旨在了解阿育吠陀方法（古印度医学体系）将个体内表型分为不同体质类型的分子决定因素，这构成了个性化治疗的基础。在本研究中，我们使用基于16S rRNA基因的微生物群落分析方法，对来自基因同质队列的三个主要群体中的健康人类肠道微生物群结构进行了探索和分析，以发现差异丰富的分类群。我们发现拟杆菌门和厚壁菌门是主要的肠道微生物成分，其组成各不相同，尽管在各类型中趋势相似。核心微生物群的多个物种在各类型中显示出差异丰度，具有性别特异性的标志性分类群。我们的研究表明，尽管肠道微生物群落的总体组成一致，但属于不同类型的健康个体中存在特定细菌的富集。它突出了基于类型的内表型对于解释健康个体肠道微生物群变异性的重要性，而这种变异性对个体的健康、疾病和治疗具有重要影响。
