Miyoshi Norikatsu, Fujino Shiki, Ohue Masayuki, Yasui Masayoshi, Takahashi Yusuke, Sugimura Keijiro, Tomokuni Akira, Akita Hirofumi, Kobayashi Shogo, Takahashi Hidenori, Omori Takeshi, Miyata Hiroshi, Yano Masahiko
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2-E2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Division of Next-generation Innovative Cancer Research and Translational Medicine, Osaka International Cancer Institute, 3-1-69, Ohtemae, Chuo-ku, Osaka, 541-8567, Japan.
Surg Today. 2018 Jul;48(7):709-715. doi: 10.1007/s00595-018-1644-9. Epub 2018 Feb 27.
The POU5F1 gene, which encodes the POU domain, class 5, transcription factor 1 (also known as Oct-4), is expressed in embryonic stem cells where it regulates pluripotency and proliferation. Few studies have examined the expression and significance of POU5F1 in cancer tissues. The aim of this study was to clarify its significance in colorectal cancer (CRC).
The study included 95 patients who underwent surgery for CRC from 2009 to 2011. The correlations between the POU5F1 gene expression and the clinical parameters were assessed in these patients. The serum TP53 expression levels were also examined by an enzyme-linked immunosorbent assay.
Patients with a high POU5F1 expression were significantly more likely to have a poor prognosis than those with a low expression. In contrast, patients with a low POU5F1 expression had a better disease-free survival after curative surgical resection than those with a high expression (P = 0.007). Multivariate analyses showed that the POU5F1 expression (P = 0.003) and lymph node metastasis (P < 0.001) were significantly correlated with the disease-free survival. The prognosis was significantly related to the serum TP53 levels, according to the POU5F1 expression.
The POU5F1 expression was suggested to be a prognostic factor in patients with CRC.
POU5F1基因编码POU结构域第5类转录因子1（也称为Oct-4），在胚胎干细胞中表达，调节多能性和增殖。很少有研究探讨POU5F1在癌组织中的表达及意义。本研究旨在阐明其在结直肠癌（CRC）中的意义。
本研究纳入了2009年至2011年接受CRC手术的95例患者。评估这些患者中POU5F1基因表达与临床参数之间的相关性。还通过酶联免疫吸附测定法检测血清TP53表达水平。
POU5F1高表达患者的预后明显比低表达患者差。相比之下，POU5F1低表达患者在根治性手术切除后的无病生存期比高表达患者更好（P = 0.007）。多因素分析显示，POU5F1表达（P = 0.003）和淋巴结转移（P < 0.001）与无病生存期显著相关。根据POU5F1表达情况，预后与血清TP53水平显著相关。
POU5F1表达被认为是CRC患者的一个预后因素。
