Department of Obstetrics and Gynecology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Int J Gynecol Cancer. 2018 May;28(4):675-683. doi: 10.1097/IGC.0000000000001201.
The ubiquitin C-terminal hydrolase L1 (UCHL1) plays a key role in tumor invasion and metastasis. Ubiquitin C-terminal hydrolase L1 is overexpressed in various cancers and reported to be correlated with a poor prognosis. The objective of this study was to determine the prognostic significance of UCHL1 in endometrial cancer.
The expression of UCHL1 in endometrial cancer was assessed using quantitative reverse transcription polymerase chain reaction and immunohistochemistry in 56 and 215 resected tumor specimens, respectively.
The 4-year survival rates of the high UCHL1 messenger RNA expression group and high UCHL1 protein expression group were 78% and 71%, respectively, compared with 96% and 95% for the low UCHL1 messenger RNA expression group and low UCHL1 protein expression group, respectively. Kaplan-Meier and log-rank tests indicated a significant correlation between expression of UCHL1 and disease-free survival and overall survival. Moreover, multivariate stepwise Cox proportional hazard regression model analysis showed that UCHL1 was a significant independent marker for predicting a poor disease-free survival and overall survival. In 43 patients with metastatic lesions, immunohistochemical analysis of metastatic lesions revealed that the recurrence rate and mortality rate were 62% and 41%, respectively, in 29 UCHL1-positive patients and 36% and 29%, respectively, in 14 UCHL1-negative patients.
The results of this study suggest that high UCHL1 expression is a strong marker of poor prognosis of endometrial cancer. Furthermore, we suggest that UCHL1 may be involved in the development of distant metastasis in endometrial cancer.
泛素 C 端水解酶 L1（UCHL1）在肿瘤侵袭和转移中发挥关键作用。UCHL1 在各种癌症中过度表达，并与预后不良相关。本研究旨在确定UCHL1 在子宫内膜癌中的预后意义。
使用定量逆转录聚合酶链反应和免疫组织化学法分别评估了 56 例和 215 例切除肿瘤标本中 UCHL1 的表达。
高 UCHL1 mRNA 表达组和高 UCHL1 蛋白表达组的 4 年生存率分别为 78%和 71%，而低 UCHL1 mRNA 表达组和低 UCHL1 蛋白表达组的生存率分别为 96%和 95%。Kaplan-Meier 和对数秩检验表明，UCHL1 的表达与无病生存率和总生存率之间存在显著相关性。此外，多变量逐步 Cox 比例风险回归模型分析表明，UCHL1 是预测无病生存率和总生存率不良的独立标志物。在 43 例有转移病灶的患者中，对转移病灶进行免疫组织化学分析显示，29 例 UCHL1 阳性患者的复发率和死亡率分别为 62%和 41%，14 例 UCHL1 阴性患者的复发率和死亡率分别为 36%和 29%。
本研究结果表明，高 UCHL1 表达是子宫内膜癌预后不良的强标志物。此外，我们认为 UCHL1 可能参与了子宫内膜癌远处转移的发生。
