Kong Tae Hoon, Yu Sunkon, Jung Byungjo, Choi Jin Sil, Seo Young Joon
Department of Otorhinolaryngology-Head and Neck Surgery, Yonsei University Wonju College of Medicine, Wonju, South Korea.
Department of Biomedical Engineering, Yonsei University College of Health Science, Wonju, South Korea.
PLoS One. 2018 Feb 28;13(2):e0191978. doi: 10.1371/journal.pone.0191978. eCollection 2018.
Laser speckle contrast imaging (LSCI) enables continuous high-resolution assessment of microcirculation in real-time. We applied an endoscope to LSCI to measure cochlear blood-flow in an ischemia-reperfusion mouse model. We also explored whether using xenon light in combination with LSCI facilitates visualization of anatomical position. Based on a previous preliminary study, the appropriate wavelength for penetrating the thin bony cochlea was 830 nm. A 2.7-mm-diameter endoscope was used, as appropriate for the size of the mouse cochlea. Our endoscopic LSCI system was used to illuminate the right cochlea after dissection of the mouse. We observed changes in the speckle signals when we applied the endoscopic LSCI system to the ischemia-reperfusion mouse model. The anatomical structure of the mouse cochlea and surrounding structures were clearly visible using the xenon light. The speckle signal of the cochlea was scattered, with an intensity that varied between that of the stapes (with the lowest signal), the negative control, and the stapedial artery (with the highest signal), the positive control. In the cochlear ischemia-reperfusion mouse model, the speckle signal of the cochlea decreased during the ischemic phase, and increased during the reperfusion phase, clearly reflecting cochlear blood-flow. The endoscopic LSCI system generates high-resolution images in real-time, allowing visualization of blood-flow and its changes in the mouse cochlea. Anatomical structures were clearly matched using LSCI along with visible light.
激光散斑对比成像（LSCI）能够实时对微循环进行连续的高分辨率评估。我们将内窥镜应用于LSCI，以测量缺血再灌注小鼠模型中的耳蜗血流。我们还探究了将氙灯与LSCI结合使用是否有助于解剖位置的可视化。基于之前的初步研究，穿透薄骨耳蜗的合适波长为830nm。使用了直径为2.7mm的内窥镜，其尺寸适合小鼠耳蜗。我们的内窥镜LSCI系统用于在解剖小鼠后照亮右侧耳蜗。当我们将内窥镜LSCI系统应用于缺血再灌注小鼠模型时，我们观察到了散斑信号的变化。使用氙灯时，小鼠耳蜗和周围结构的解剖结构清晰可见。耳蜗的散斑信号是分散的，其强度在镫骨（信号最低）、阴性对照和镫骨动脉（信号最高）、阳性对照之间变化。在耳蜗缺血再灌注小鼠模型中，耳蜗的散斑信号在缺血期降低，在再灌注期升高，清楚地反映了耳蜗血流。内窥镜LSCI系统实时生成高分辨率图像，能够可视化小鼠耳蜗中的血流及其变化。使用LSCI结合可见光可以清晰地匹配解剖结构。
