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长链非编码 RNA SPRY4-IT1 通过调控胰腺癌细胞中的 Cdc20 促进细胞增殖和侵袭。

Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells.

机构信息

Department of Abdominal Oncology, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan Province, People's Republic of China.

Lab of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

出版信息

PLoS One. 2018 Feb 28;13(2):e0193483. doi: 10.1371/journal.pone.0193483. eCollection 2018.

DOI:10.1371/journal.pone.0193483
PMID:29489909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5831108/
Abstract

Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play a critical role in the development of human cancers including pancreatic cancer. Long non-coding RNA SPRY4-IT1 (sprouty4-intron transcript 1) has been reported to play an oncogenic role in various types of human carcinomas. However, the role of SPRY4-IT1 in pancreatic cancer is unclear. The objective of this study was to determine the function of SPRY4-IT1 on proliferation and invasion in pancreatic cancer. In the current study, we dissected the function and mechanism of SPRY4-IT1 by multiple approaches including Real-time RT-PCR, Western blotting analysis, MTT assay, Wound healing assay, Transwell assay, and transfection. We found that down-regulation of SPRY4-IT1 inhibited cell growth and induced cell apoptosis in pancreatic cancer cells. Moreover, SPRY4-IT1 knockdown induced cell cycle arrest at G0/G1 phase. Furthermore, inhibition of SPRY4-IT1 retarded cell migration and invasion in pancreatic cancer cells. Overexpression of SPRY4-IT1 enhanced cell growth and invasion, and inhibited cell apoptosis in pancreatic cancer cells. Mechanistically, suppression of SPRY4-IT1 inhibited the expression of Cdc20 in pancreatic cancer cells. Our findings demonstrated that inhibition of SPRY4-IT1 could be a potential therapeutic approach for the treatment of pancreatic cancer.

摘要

越来越多的证据表明，长非编码 RNA（lncRNA）在人类癌症的发展中起着关键作用，包括胰腺癌。长非编码 RNA SPRY4-IT1（sprouty4-内含子转录本 1）已被报道在各种人类癌中发挥致癌作用。然而，SPRY4-IT1 在胰腺癌中的作用尚不清楚。本研究旨在确定 SPRY4-IT1 在胰腺癌中的增殖和侵袭功能。在本研究中，我们通过多种方法，包括实时 RT-PCR、Western blot 分析、MTT 测定、划痕愈合测定、Transwell 测定和转染，来剖析 SPRY4-IT1 的功能和机制。我们发现下调 SPRY4-IT1 抑制了胰腺癌细胞的生长并诱导了细胞凋亡。此外，SPRY4-IT1 敲低诱导细胞周期停滞在 G0/G1 期。此外，抑制 SPRY4-IT1 可减缓胰腺癌细胞的迁移和侵袭。SPRY4-IT1 的过表达增强了胰腺癌细胞的生长和侵袭，并抑制了细胞凋亡。从机制上讲，抑制 SPRY4-IT1 可抑制胰腺癌细胞中 Cdc20 的表达。我们的研究结果表明，抑制 SPRY4-IT1 可能是治疗胰腺癌的一种潜在治疗方法。

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