Moringo Nicholas A, Shen Hao, Bishop Logan D C, Wang Wenxiao, Landes Christy F
Department of Chemistry, Rice University, Houston, Texas 77251, USA; email:
Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77251, USA.
Annu Rev Phys Chem. 2018 Apr 20;69:353-375. doi: 10.1146/annurev-physchem-052516-045018. Epub 2018 Feb 28.
Super-resolution microscopy is becoming an invaluable tool to investigate structure and dynamics driving protein interactions at interfaces. In this review, we highlight the applications of super-resolution microscopy for quantifying the physics and chemistry that occur between target proteins and stationary-phase supports during chromatographic separations. Our discussion concentrates on the newfound ability of super-resolved single-protein spectroscopy to inform theoretical parameters via quantification of adsorption-desorption dynamics, protein unfolding, and nanoconfined transport.
超分辨率显微镜正成为研究界面处驱动蛋白质相互作用的结构和动力学的一项宝贵工具。在本综述中，我们重点介绍超分辨率显微镜在量化色谱分离过程中目标蛋白质与固定相载体之间发生的物理和化学过程方面的应用。我们的讨论集中在超分辨单蛋白光谱通过量化吸附 - 解吸动力学、蛋白质解折叠和纳米受限传输来提供理论参数的新发现能力上。
