Cheng Xiaoyu, Yin Wei
State Key Laboratory for Modern Optical Instrumentations, National Engineering Research Center of Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Core Facilities, School of Medicine, Zhejiang University, Hangzhou, China.
Front Chem. 2021 Apr 29;9:655324. doi: 10.3389/fchem.2021.655324. eCollection 2021.
Far field single molecule localization microscopy (SMLM) has been established as a powerful tool to study biological structures with resolution far below the diffraction limit of conventional light microscopy. In recent years, the applications of SMLM have reached beyond traditional cellular imaging. Nanostructured interfaces are enriched with information that determines their function, playing key roles in applications such as chemical catalysis and biological sensing. SMLM enables detailed study of interfaces at an individual molecular level, allowing measurements of reaction kinetics, and detection of rare events not accessible to ensemble measurements. This paper provides an update to the progress made to the use of SMLM in characterizing nanostructured biointerfaces, focusing on practical aspects, recent advances, and emerging opportunities from an analytical chemistry perspective.
远场单分子定位显微镜（SMLM）已成为一种强大的工具，用于研究生物结构，其分辨率远低于传统光学显微镜的衍射极限。近年来，SMLM的应用已超越传统的细胞成像。纳米结构界面富含决定其功能的信息，在化学催化和生物传感等应用中发挥着关键作用。SMLM能够在单个分子水平上对界面进行详细研究，从而实现反应动力学的测量，并检测整体测量无法获取的罕见事件。本文从分析化学的角度，介绍了SMLM在表征纳米结构生物界面方面取得的进展，重点关注实际应用、最新进展和新出现的机会。
