Lin Liulan, Wang Tianjiang, Zhou Qi, Qian Niandong
Rapid Manufacture Engineering Center, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, China.
Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Des Monomers Polym. 2016 Nov 28;20(1):351-362. doi: 10.1080/15685551.2016.1259839. eCollection 2017.
OIC-A006 (BMPs osteogenesis compounds), can stimulate bone marrow mesenchymal stem cells ALP, OPN, OC, Cbfal expression. To stimulate new bone formation in the body. We postulate different amounts of drug microspheres on the PLGA/β-CPT scaffold can produce the effects on performance and sustained release characteristics. In this paper, through adding different amount of carrier drug microsphere, three concentrations scaffolds which are 12.5, 18.75 and 25 μmol/L are prepared by adding different amounts of drug-loaded microspheres. Hereafter called OICM/CPT-200, OICM/CPT-300, OICM/CPT-400. We implant them in rat femur diameter 3 mm depth of 3 mm hole for eight weeks. The degradation, microsphere, delivery properties, with X-ray, micro-CT and histology are tested. Results show that the contain carrier drug microsphere scaffolds become radiopaque, and the gaps between the scaffold and radial cut ends are often invisible. This preliminary study reveals that different carrier drug microsphere has a corresponding effect the performance of stent body, OICM/CPT - 200 scaffolds induction effect is best. Illustrates that the low concentration load OIC-A006 microspheres can promote bone healing, and high concentration of OIC-A006 micro ball is played a inhibitory effect on bone healing process.
OIC-A006（骨形态发生蛋白成骨化合物）可刺激骨髓间充质干细胞碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OC）、核心结合因子α1（Cbfal）的表达，以刺激体内新骨形成。我们推测，在聚乳酸-羟基乙酸共聚物/β-磷酸三钙（PLGA/β-CPT）支架上负载不同量的药物微球会对其性能和缓释特性产生影响。本文通过添加不同量的载药微球，制备了浓度分别为12.5、18.75和25μmol/L的三种浓度支架，分别命名为OICM/CPT-200、OICM/CPT-300、OICM/CPT-400。将其植入大鼠股骨直径3mm、深度3mm的骨洞中8周，通过X射线、显微CT和组织学检测其降解、微球及释放特性。结果显示，含载药微球的支架变得不透射线，支架与骨切面之间的间隙往往不可见。这项初步研究表明，不同的载药微球对支架主体性能有相应影响，OICM/CPT - 200支架的诱导效果最佳。说明低浓度负载OIC-A006微球可促进骨愈合，而高浓度的OIC-A006微球对骨愈合过程起抑制作用。
