来自原发性HIV-1样本的完整基因快速测序
Rapid Sequencing of Complete Genes from Primary HIV-1 Samples.
作者信息
Laird Smith Melissa, Murrell Ben, Eren Kemal, Ignacio Caroline, Landais Elise, Weaver Steven, Phung Pham, Ludka Colleen, Hepler Lance, Caballero Gemma, Pollner Tristan, Guo Yan, Richman Douglas, Poignard Pascal, Paxinos Ellen E, Kosakovsky Pond Sergei L, Smith Davey M
机构信息
Pacific Biosciences, Menlo Park, CA, USA.
Department of Medicine, University of California, San Diego, CA, USA.
出版信息
Virus Evol. 2016 Jul 8;2(2):vew018. doi: 10.1093/ve/vew018. eCollection 2016 Jul.
Abstract
The ability to study rapidly evolving viral populations has been constrained by the read length of next-generation sequencing approaches and the sampling depth of single-genome amplification methods. Here, we develop and characterize a method using Pacific Biosciences' Single Molecule, Real-Time (SMRT®) sequencing technology to sequence multiple, intact full-length human immunodeficiency virus-1 genes amplified from viral RNA populations circulating in blood, and provide computational tools for analyzing and visualizing these data.
摘要
研究快速进化的病毒群体的能力一直受到下一代测序方法的读长以及单基因组扩增方法的采样深度的限制。在此，我们开发并表征了一种使用太平洋生物科学公司的单分子实时（SMRT®）测序技术对从血液中循环的病毒RNA群体中扩增出的多个完整全长人类免疫缺陷病毒1型基因进行测序的方法，并提供了用于分析和可视化这些数据的计算工具。
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