Giampetruzzi Annalisa, Chiumenti Michela, Minafra Angelantonio, Saldarelli Pasquale
Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari "Aldo Moro", Bari, Italy.
Istituto per la Protezione Sostenibile delle Piante del CNR, Bari, Italy.
Methods Mol Biol. 2018;1746:27-36. doi: 10.1007/978-1-4939-7683-6_2.
A protocol is described to purify small (s)RNA molecules from tissues of grapevine and other woody plants. The protocol has been specifically developed to analyze sRNA populations by high-throughput sequencing. It has been widely used on species of the genera Prunus and Vitis particularly rich in polyphenols and other enzyme-inhibiting compounds. The high quality of the sRNAs extracted from leaf or phloem tissues makes them suitable for all molecular biology reactions, in particular for next-generation sequencing library preparation.
本文描述了一种从葡萄和其他木本植物组织中纯化小（s）RNA分子的方法。该方法是专门为通过高通量测序分析sRNA群体而开发的。它已广泛应用于特别富含多酚和其他酶抑制化合物的李属和葡萄属物种。从叶片或韧皮部组织中提取的高质量sRNA使其适用于所有分子生物学反应，特别是用于下一代测序文库的制备。
