Institute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, Italy.
Dipartimento di Scienze del suolo, della Pianta e degli Alimenti, University of Bari "Aldo Moro", Via Amendola, 165/A, 70126 Bari, Italy.
Viruses. 2021 Mar 15;13(3):481. doi: 10.3390/v13030481.
In 2014, high-throughput sequencing of libraries of total DNA from olive trees allowed the identification of two geminivirus-like contigs. After conventional resequencing of the two genomic DNAs, their analysis revealed they belonged to the same viral entity, for which the provisional name of Olea europaea geminivirus (OEGV) was proposed. Although DNA-A showed a genome organization similar to that of New World begomoviruses, DNA-B had a peculiar ORF arrangement, consisting of a movement protein (MP) in the virion sense and a protein with unknown function on the complementary sense. Phylogenetic analysis performed either on full-length genome or on coat protein, replication associated protein (Rep), and MP sequences did not endorse the inclusion of this virus in any of the established genera in the family . A survey of 55 plants revealed that the virus is widespread in Apulia (Italy) with 91% of the samples testing positive, although no correlation of OEGV with a disease or specific symptoms was encountered. Southern blot assay suggested that the virus is not integrated in the olive genome. The study of OEGV-derived siRNA obtained from small RNA libraries of leaves and fruits of three different cultivars, showed that the accumulation of the two genomic components is influenced by the plant genotype while virus-derived-siRNA profile is in line with other geminivirids reported in literature. Single-nucleotide polymorphism (SNP) analysis unveiled a low intra-specific variability.
2014 年，对橄榄树总 DNA 文库进行高通量测序，鉴定出了两个类似双生病毒的序列。对两个基因组 DNA 进行常规重测序后，分析表明它们属于同一病毒实体，暂定名为油橄榄双生病毒（OEGV）。虽然 DNA-A 的基因组组织与新世界伴随病毒相似，但 DNA-B 的 ORF 排列具有独特性，其在病毒 sense 上具有运动蛋白（MP），在互补 sense 上具有未知功能的蛋白。无论是基于全基因组序列还是衣壳蛋白、复制相关蛋白（Rep）和 MP 序列进行的系统发育分析，都不支持将该病毒归入家族中已建立的任何属中。对 55 株植物的调查显示，该病毒在意大利普利亚广泛存在，91%的样本呈阳性，但未发现 OEGV 与任何疾病或特定症状有关。Southern blot 分析表明，该病毒未整合到橄榄基因组中。对来自三个不同品种的叶片和果实小 RNA 文库中获得的 OEGV 衍生 siRNA 的研究表明，两种基因组成分的积累受植物基因型的影响，而病毒衍生的 siRNA 谱与文献中报道的其他双生病毒一致。单核苷酸多态性（SNP）分析揭示了低的种内变异性。
