State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling 712100, China.
Int J Mol Sci. 2018 Mar 1;19(3):696. doi: 10.3390/ijms19030696.
Ethylene response factor (ERF) transcription factors play important roles in regulating immune responses in plants. In our study, we characterized a member of the ERF transcription factor family, , from the Chinese wild genotype, Rupr "Shuangyou". Phylogenetic analysis indicated that belongs to group IXc of the ERF family, in which many members are known to contribute to fighting pathogen infection. Consistent with this, expression of was induced by treatment with the necrotrophic fungal pathogen ) in "Shuangyou" and "Red Globe". plants over-expressing displayed enhanced resistance to and the bacterium pv. tomato () DC3000. Patterns of pathogen-induced reactive oxygen species (ROS) accumulation were entirely distinct in and DC3000 inoculated plants. Examples of both salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) responsive defense genes were up-regulated after and DC3000 inoculation of the -overexpressing transgenic plants. Evidence of pattern-triggered immunity (PTI), callose accumulation and stomatal defense, together with increased expression of PTI genes, was also greater in the transgenic lines. These data indicate that participates in various signal transduction pathways and acts as an inducer of immune responses.
乙烯应答因子（ERF）转录因子在植物免疫反应的调控中发挥着重要作用。在本研究中，我们对来自中国野生型基因型‘双优’的 ERF 转录因子家族的一个成员进行了特征描述。系统进化分析表明，属于 ERF 家族的第 IXc 组，其中许多成员被认为有助于抵御病原体感染。与这一观点一致的是，在‘双优’和‘Red Globe’中，受到坏死真菌病原体（）处理后，表达。过量表达的植株对和细菌 pv. tomato () DC3000 表现出增强的抗性。在和 DC3000 接种的植株中，病原体诱导的活性氧（ROS）积累模式完全不同。在接种和 DC3000 后，水杨酸（SA）和茉莉酸/乙烯（JA/ET）响应防御基因的表达均上调。在转基因植株中，还观察到模式触发免疫（PTI）、胼胝质积累和气孔防御的证据，以及 PTI 基因的表达增加。这些数据表明，参与了各种信号转导途径，并作为免疫反应的诱导剂。
