College of Animal Science and Technology, Northwest A&F University, 712100 Yangling, Shaanxi, P.R. China.
Animal Science, School of Agriculture and Environment, Massey University, Palmerston North, New Zealand.
Genome. 2020 Oct;63(10):483-492. doi: 10.1139/gen-2019-0184. Epub 2020 Jul 2.
The gene produces a phosphorylated protein wrapped in lipid droplets in adipocytes. This phosphorylation assists the mobilization of fat into adipose tissue. The purpose of the experiment was to study the polymorphism of the gene and its relationship with the body and carcass characteristics of Qinchuan cattle to find molecular genetic markers that can be used for breeding. The expression level of the gene was determined in various tissues by qRT-PCR. The results showed that the highest level of expression was found in subcutaneous fat, followed by the heart and longissimus muscle, and the lowest level was found in the kidney. Five SNP loci of the gene were identified in 510 Qinchuan cattle, including g.3580T>C (SNP1), g.3898G>A (SNP2), g.8333G>A (SNP3), g.10517T>C (SNP4), and g.10538G>T (SNP5). The results show that SNP1, SNP2, SNP3, and SNP4 were moderately polymorphic (0.25 < PIC < 0.5), while SNP5 was minimally polymorphic (PIC < 0.25). SNP2, SNP3, and SNP5 were within Hardy-Weinberg equilibrium ( > 0.05), but SNP1 and SNP4 were not ( < 0.05). Correlation analysis showed that the five SNPs of the gene were correlated with back-fat depth, intramuscular fat, and chest depth of Qinchuan cattle. The double haplotype H2H4 in Qinchuan beef was associated with body and carcass traits. We conclude that variants mapped within can be used in marker-assisted selection for carcass quality and body traits in breed improvement programs for Qinchuan cattle.
该基因产生一种被脂肪滴包裹的磷酸化蛋白质。这种磷酸化有助于脂肪向脂肪组织的动员。该实验的目的是研究 基因的多态性及其与秦川牛体躯和胴体特征的关系，以寻找可用于育种的分子遗传标记。通过 qRT-PCR 测定了该基因在各种组织中的表达水平。结果表明，的表达水平最高的是皮下脂肪，其次是心脏和背最长肌，最低的是肾脏。在 510 头秦川牛中鉴定出 基因的 5 个 SNP 位点，包括 g.3580T>C（SNP1）、g.3898G>A（SNP2）、g.8333G>A（SNP3）、g.10517T>C（SNP4）和 g.10538G>T（SNP5）。结果表明，SNP1、SNP2、SNP3 和 SNP4 为中度多态性（0.25<PIC<0.5），而 SNP5 为低度多态性（PIC<0.25）。SNP2、SNP3 和 SNP5 处于 Hardy-Weinberg 平衡状态（>0.05），但 SNP1 和 SNP4 没有（<0.05）。相关性分析表明，该基因的五个 SNP 与秦川牛背膘厚、肌内脂肪和胸深相关。秦川牛肉的双单倍型 H2H4 与体躯和胴体性状相关。我们得出结论，在秦川牛的育种改良计划中，可将位于 内的变异用于辅助选择胴体质量和体躯性状。
