College of Pharmacy, Heze University, Heze, Shandong Province, 274000, People's Republic of China.
Gaoqing County Black Cattle Industry Development Center, Gaoqing County Bureau of Agriculture and Rural Affairs, Zibo, Shandong Province, 255000, People's Republic of China.
Trop Anim Health Prod. 2023 Dec 18;56(1):19. doi: 10.1007/s11250-023-03858-z.
In the current study, the role of the ovine IGF2 as a potential candidate gene was investigated as though marker-assisted selection in Chinese Tibetan sheep. The Sanger DNA sequencing method explored five single nucleotide polymorphisms (SNPs) in 5'UTR of the ovine IGF2 gene (C15640T, G15801A, G15870A, C15982G and G15991A) in Chinese Tibetan sheep. The frequencies of four SNPs were within the Hardy-Weinberg Equilibrium (chi-square test) except C15982G. The statistical analysis indicated that the C15640T and G15801A were significantly associated with body height, body length, chest circumference, and body weight (P < 0.05 or P < 0.01). Furthermore, C15982G variant exhibited significant correlation with the body weight (P < 0.01). These findings suggests that the promoter variants of IGF2 gene could be used as a candidate gene through marker-assisted selection for the body weight and body measurement traits in Tibetan sheep breeding program.
在本研究中，尽管对中国藏绵羊进行了标记辅助选择，但研究了绵羊 IGF2 作为潜在候选基因的作用。通过 Sanger DNA 测序方法，在绵羊 IGF2 基因 5'UTR 中探索了五个单核苷酸多态性（SNPs）（C15640T、G15801A、G15870A、C15982G 和 G15991A）。除 C15982G 外，四个 SNPs 的频率均处于哈迪-温伯格平衡（卡方检验）。统计分析表明，C15640T 和 G15801A 与体高、体长、胸围和体重显著相关（P<0.05 或 P<0.01）。此外，C15982G 变异与体重显著相关（P<0.01）。这些发现表明，IGF2 基因启动子变异可作为候选基因，通过标记辅助选择用于藏绵羊体重和体尺性状的选育计划。
