Italian Foundation for Cancer Research, the Fondazione Italiana per la Ricerca sul Cancro Institute of Molecular Oncology, Milan, Italy
Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Pavia, Italy.
J Cell Biol. 2018 Apr 2;217(4):1177-1179. doi: 10.1083/jcb.201802083. Epub 2018 Mar 1.
Roy et al. (2018. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations.
罗伊等人(2018 年。https://doi.org/10.1083/jcb.201709121)描述了一种巧妙的单细胞分析系统,即 DNA 复制叉处蛋白质相互作用的原位分析(SIRF),用于定量分析活跃和停滞的复制叉处新生 DNA 上的蛋白质相互作用。灵敏准确的 SIRF 方法适用于细胞群体中的多参数测量。