Cole S, Fairweather J M, Townsend K M
Br J Dermatol. 1987 Jan;116(1):55-65. doi: 10.1111/j.1365-2133.1987.tb05791.x.
Langerhans cell numbers, morphology and distribution were observed in cross sections of footpad epidermis at intervals from 1 to 28 days after exposure of the hind feet of CBA/H mice or albino guinea-pigs to a single absorbed dose of 20 Gy (2000 rad) of X-rays. In mice, the number of Langerhans cells reactive with anti-macrophage F4/80 monoclonal antibody steadily declined by approximately 85% within 10 days after irradiation, consistent with previous studies, in which Langerhans cells were identified in epidermal sheets by ATPase activity or presence of Birbeck granules. Remaining Langerhans cells were exceptionally dendritic. Very few Birbeck granule-containing cells were found in murine popliteal lymph nodes before or after irradiation but damaged cells were present in superficial strata of irradiated epidermis. The morphology and number of epidermal F4/80-positive cells approached normal by 15 days after irradiation. In guinea-pigs, gradual suprabasal movement and loss of rounded, ATPase-positive Langerhans cells from the epidermis were detectable from 5 to 20 days after irradiation but the magnitude of the cell loss and redistribution was partially obscured by the simultaneous appearance of clusters of replacement Langerhans cells in the basal layer and by keratinocyte hyperplasia.
在CBA/H小鼠或白化豚鼠的后足接受单次20 Gy（2000拉德）X射线吸收剂量照射后的1至28天内，每隔一段时间观察足垫表皮横切面中的朗格汉斯细胞数量、形态和分布。在小鼠中，与抗巨噬细胞F4/80单克隆抗体反应的朗格汉斯细胞数量在照射后10天内稳步下降约85%，这与之前的研究一致，在之前的研究中，通过ATP酶活性或伯贝克颗粒的存在在表皮片中鉴定出朗格汉斯细胞。剩余的朗格汉斯细胞具有异常的树突状形态。在照射前后，在小鼠腘窝淋巴结中很少发现含有伯贝克颗粒的细胞，但在照射后的表皮浅层存在受损细胞。照射后15天，表皮F4/80阳性细胞的形态和数量接近正常。在豚鼠中，照射后5至20天可检测到圆形的、ATP酶阳性的朗格汉斯细胞从表皮逐渐向上移动并丢失，但由于基底层中替代朗格汉斯细胞簇的同时出现以及角质形成细胞增生，细胞丢失和重新分布的程度部分被掩盖。
