Cole S, Townsend K M
Radiat Res. 1985 Aug;103(2):251-9.
Effects of a range (2-50 Gy) of single doses of 250 kV X rays on epidermal Langerhans cells in vivo were quantified in groups of CBA/CaH mice. Animals were sacrificed and compared with controls on the 10th day after local irradiation of their hind feet, when Langerhans cell numbers were at a minimum. ATPase-positive Langerhans cells in sheets of footpad epidermis were counted by light microscopy and cells with Birbeck granules were enumerated by electron microscopy. Both methods revealed a dose-dependent loss of Langerhans cells after ionizing radiation. Fifty percent of the ATPase-positive cells were lost after 14.4 +/- 1.3 Gy, and 50% of Birbeck granule-containing cells were lost after 17.9 +/- 4.2 Gy, suggesting that differentiated epidermal Langerhans cells are radioresistant. Loss of equivalent proportions of ATPase-positive and ultrastructurally identifiable cells after a range of doses indicates that X rays do not merely alter Langerhans cell surface markers but actually deplete the epidermal population of these cells.
对CBA/CaH小鼠组进行体内实验，定量研究了250 kV X射线单剂量范围（2 - 50 Gy）对表皮朗格汉斯细胞的影响。在对动物后足进行局部照射后的第10天（此时朗格汉斯细胞数量最少），将动物处死并与对照组进行比较。通过光学显微镜对足垫表皮片中ATP酶阳性的朗格汉斯细胞进行计数，通过电子显微镜对含有伯贝克颗粒的细胞进行计数。两种方法均显示电离辐射后朗格汉斯细胞呈剂量依赖性减少。14.4±1.3 Gy照射后，50%的ATP酶阳性细胞丢失；17.9±4.2 Gy照射后，50%的含伯贝克颗粒的细胞丢失，这表明分化的表皮朗格汉斯细胞具有放射抗性。在一定剂量范围内，ATP酶阳性细胞和超微结构可识别细胞的等量比例丢失，表明X射线不仅改变了朗格汉斯细胞表面标志物，实际上还耗尽了这些细胞的表皮群体。
