Cole S
J Invest Dermatol. 1986 Nov;87(5):608-12. doi: 10.1111/1523-1747.ep12455853.
Langerhans cells (LC) were studied with ADPase histochemistry in sheets of hind footpad epidermis from groups of CBA/H mice. Single, local 20-Gy doses of 250-kV x-rays were administered to the right hind feet of the mice when they were 3-4 months old, and LC were counted at intervals ranging from 2 to 24 months later. In unirradiated mice, aged 5-19 months, the mean density of LC in footpad was 1521-1617 cells/mm2. It dropped to 1137 +/- 86 cells/mm2 (mean +/- SE) in untreated 28-month-old mice. At times from 2-15 months after irradiation, normal mean densities of LC were present in footpad epidermis. On average, LC numbers were subsequently reduced to 1078 +/- 65/mm2 by 19 months after irradiation (71% of the cells in age-matched controls) and to 789 +/- 53/mm2 by 24 months (59% of the cells in age-matched controls). Loss of cells was focal. Chronic radiation-induced fibrosis and damage to circulatory function in skin may have contributed to impaired replacement of LC from bone marrow precursors. The possibility that late radiation-related depletion of the LC population permits development of skin tumors as a delayed consequence of exposure to ionizing radiation is discussed.
运用腺苷三磷酸酶（ADPase）组织化学方法，对CBA/H小鼠组后足垫表皮片层中的朗格汉斯细胞（LC）进行了研究。当小鼠3 - 4月龄时，对其右后足给予单次局部20 Gy的250 kV X射线照射，在照射后2至24个月的不同时间点对LC进行计数。在5 - 19月龄未照射的小鼠中，足垫中LC的平均密度为1521 - 1617个细胞/mm²。在未经处理的28月龄小鼠中，该密度降至1137±86个细胞/mm²（平均值±标准误）。在照射后2至15个月的各个时间点，足垫表皮中LC的平均密度均处于正常水平。平均而言，照射后19个月时，LC数量随后降至1078±65/mm²（为年龄匹配对照组细胞数量的71%），到24个月时降至789±53/mm²（为年龄匹配对照组细胞数量的59%）。细胞丢失是局灶性的。慢性辐射诱导的纤维化以及皮肤循环功能损伤可能导致了骨髓前体细胞对LC的替代受损。文中讨论了LC群体后期与辐射相关的耗竭是否会使皮肤肿瘤作为电离辐射暴露的延迟后果而发生发展的可能性。
