Department of Clinical Medicine and Surgery, Section of Dermatology, University of Naples Federico II, Naples, Italy.
Department of Medicine, Surgery and Dentistry, "Scuola Medica Salernitana" University of Salerno, Salerno, Italy.
Exp Dermatol. 2018 Jun;27(6):681-684. doi: 10.1111/exd.13521. Epub 2018 Apr 10.
In this study, we investigated the role of IL-26 in allergic contact dermatitis (ACD), highlighting its' contribute in the cytotoxic mechanism responsible for the tissue injury. IL-26 is a signature Th17 cytokine, and immune cells are its predominant sources. Recently, it has shown that Th17 cell-derived-IL-26 functions like an antimicrobial peptide. Here, we hypothesized that IL-26 could be involved in cytotoxicity mechanism that underlies ACD. Indeed, we have attributed a role to IL-26 in this context, through PBMC cytotoxicity assays vs HaCat. To demonstrate that IL-26 was effectively involved in this activity, we performed the assay using transfected ACD PBMCs by siRNA for IL-26. Indeed, we demonstrated that these cells were less able to kill keratinocytes compared with ACD PBMCs (P < .01). In conclusion, our findings support the idea that this emergent cytokine, IL-26, is implicated in the killing mechanisms of KC observed during ACD.
在这项研究中，我们研究了白细胞介素-26（IL-26）在过敏性接触性皮炎（ACD）中的作用，强调了其在导致组织损伤的细胞毒性机制中的作用。IL-26 是一种特征性的 Th17 细胞因子，免疫细胞是其主要来源。最近，研究表明 Th17 细胞衍生的 IL-26 具有抗菌肽的功能。在这里，我们假设 IL-26 可能参与了 ACD 潜在的细胞毒性机制。事实上，我们通过 PBMC 对 HaCat 的细胞毒性测定，在这方面归因于 IL-26 的作用。为了证明 IL-26 确实参与了这种活性，我们使用 siRNA 转染 ACD PBMC 进行了测定。事实上，我们证明与 ACD PBMC 相比，这些细胞对角质形成细胞的杀伤能力降低（P<.01）。总之，我们的研究结果支持这样一种观点，即这种新兴的细胞因子 IL-26 参与了 ACD 期间观察到的 KC 杀伤机制。
