Neis Mark M, Peters Bettina, Dreuw Alexandra, Wenzel Joerg, Bieber Thomas, Mauch Cornelia, Krieg Thomas, Stanzel Sven, Heinrich Peter C, Merk Hans F, Bosio Andreas, Baron Jens M, Hermanns Heike M
Department of Dermatology and Allergology, University Hospital RWTH Aachen, Germany.
J Allergy Clin Immunol. 2006 Oct;118(4):930-7. doi: 10.1016/j.jaci.2006.07.015. Epub 2006 Sep 1.
IL-31 is produced by activated T lymphocytes, preferentially by TH2 cells. Transgenic mice overexpressing IL-31 have a phenotype resembling allergic dermatitis in human subjects.
We sought to evaluate the potential importance of IL-31 in the pathogenesis of human T cell-mediated skin diseases.
We analyzed total RNA taken from 149 skin biopsy specimens from patients with atopic dermatitis (AD), allergic contact dermatitis (ACD), or psoriasis in comparison with specimens taken from patients with healthy skin (n = 13) by using quantitative real-time PCR for the expression of TH1/TH2 cytokines.
We found statistically increased mRNA levels of IL-31 in biopsy specimens taken from patients with AD, irrespective of the severity of the disease and serum IgE levels. Moreover, IL-31 mRNA levels were strongly increased in many biopsy specimens taken from patients with ACD. However, no increased transcription of IL-31 could be detected in biopsy specimens taken from psoriatic plaques. A comparison of mRNA levels of IL-31 with TH1 or TH2 cytokines demonstrates a correlation of the expression of IL-31 with IL-4 and IL-13 but not with IFN-gamma. No significant increase of IL-31 receptor mRNA could be detected in any disease, whereas the second receptor subunit of IL-31, the oncostatin M receptor, seems to be enhanced transcribed in patients with psoriasis.
IL-31 expression is not only increased in patients with AD but also in those with ACD, 2 pruritic skin disorders. In both types of eczema, expression of IL-31 is associated with the expression of the TH2 cytokines IL-4 and IL-13.
IL-31 might contribute not only to the development of AD but also to ACD-provoked skin inflammation.
IL-31由活化的T淋巴细胞产生，主要由TH2细胞产生。过表达IL-31的转基因小鼠具有类似于人类过敏性皮炎的表型。
我们试图评估IL-31在人类T细胞介导的皮肤疾病发病机制中的潜在重要性。
我们通过定量实时PCR分析了149例特应性皮炎（AD）、过敏性接触性皮炎（ACD）或银屑病患者的皮肤活检标本中的总RNA，并与13例健康皮肤患者的标本进行比较，以检测TH1/TH2细胞因子的表达。
我们发现，无论疾病严重程度和血清IgE水平如何，AD患者活检标本中IL-31的mRNA水平在统计学上均升高。此外，许多ACD患者的活检标本中IL-31 mRNA水平也显著升高。然而，在银屑病斑块的活检标本中未检测到IL-31转录增加。将IL-31的mRNA水平与TH1或TH2细胞因子进行比较，结果显示IL-31的表达与IL-4和IL-13相关，但与IFN-γ无关。在任何疾病中均未检测到IL-31受体mRNA的显著增加，而IL-31的第二个受体亚基，即抑瘤素M受体，在银屑病患者中似乎转录增强。
IL-31不仅在AD患者中表达增加，在ACD患者中也增加，这是两种瘙痒性皮肤病。在这两种类型的湿疹中，IL-31的表达均与TH2细胞因子IL-4和IL-13的表达相关。
IL-31可能不仅有助于AD的发生，也有助于ACD引发的皮肤炎症。
