The University of Akron, Olson Research Center 319, 302 E. Buchtel Ave., Akron 44325-0302, OH, USA.
Exp Cell Res. 2018 Apr 1;365(1):106-118. doi: 10.1016/j.yexcr.2018.02.027. Epub 2018 Feb 28.
Researchers have been using lab-on-a-chip systems to isolate factors for study, simulate laboratory analysis and model cellular, tissue and organ level processes. The technology is increasing rapidly, but the bone field has been slow to keep pace. Novel models are needed that have the power and flexibility to investigate the elegant and synchronous multicellular interactions that occur in normal bone turnover and in disease states in which remodeling is implicated. By removing temporal and spatial limitations and enabling quantification of functional outcomes, the platforms should provide unique environments that are more biomimetic than single cell type systems while minimizing complex systemic effects of in vivo models. This manuscript details the development and characterization of lab-on-a-chip platforms for stimulating osteocytes and quantifying bone remodeling. Our platforms provide the foundation for a model that can be used to investigate remodeling interactions as a whole or as a standard mechanotransduction tool by which isolated activity can be quantified as a function of load.
研究人员一直在使用芯片实验室系统来分离因素进行研究，模拟实验室分析和细胞、组织和器官水平的过程建模。该技术发展迅速，但骨骼领域一直难以跟上步伐。需要有强大和灵活的新型模型来研究正常骨转换和涉及重塑的疾病状态下发生的优雅和同步的多细胞相互作用。通过消除时间和空间限制并能够量化功能结果，这些平台应该提供比单细胞类型系统更仿生的独特环境，同时最小化体内模型的复杂系统效应。本文详细介绍了用于刺激成骨细胞和量化骨重塑的芯片实验室平台的开发和特性。我们的平台为模型的发展奠定了基础，该模型可用于整体研究重塑相互作用，也可作为标准的机械转导工具，根据负载来量化分离的活性。
