南美和非洲锥虫 vivax 株的比较计算机线性 B 细胞表位预测和特征分析。
A comparative in silico linear B-cell epitope prediction and characterization for South American and African Trypanosoma vivax strains.
机构信息
Laboratório Nacional de Computação Científica (LNCC), Av. Getúlio Vargas, 333, Petrópolis, RJ, Brazil; Grupo Hermes Pardini, Setor de Pesquisa e Desenvolvimento, Vespasiano, MG, Brazil.
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil.
出版信息
Genomics. 2019 May;111(3):407-417. doi: 10.1016/j.ygeno.2018.02.017. Epub 2018 Feb 27.
Trypanosoma vivax is a parasite widespread across Africa and South America. Immunological methods using recombinant antigens have been developed aiming at specific and sensitive detection of infections caused by T. vivax. Here, we sequenced for the first time the transcriptome of a virulent T. vivax strain (Lins), isolated from an outbreak of severe disease in South America (Brazil) and performed a computational integrated analysis of genome, transcriptome and in silico predictions to identify and characterize putative linear B-cell epitopes from African and South American T. vivax. A total of 2278, 3936 and 4062 linear B-cell epitopes were respectively characterized for the transcriptomes of T. vivax LIEM-176 (Venezuela), T. vivax IL1392 (Nigeria) and T. vivax Lins (Brazil) and 4684 for the genome of T. vivax Y486 (Nigeria). The results presented are a valuable theoretical source that may pave the way for highly sensitive and specific diagnostic tools.
布氏锥虫广泛分布于非洲和南美洲。已经开发出使用重组抗原的免疫学方法，旨在针对由 T. vivax 引起的感染进行特异性和敏感性检测。在这里，我们首次对来自南美洲（巴西）严重疾病爆发的一株毒力 T. vivax 株（Lins）进行了转录组测序，并对基因组、转录组和计算机预测进行了综合分析，以鉴定和表征来自非洲和南美洲 T. vivax 的潜在线性 B 细胞表位。分别对来自委内瑞拉的 T. vivax LIEM-176、尼日利亚的 T. vivax IL1392 和巴西的 T. vivax Lins 的转录组进行了 2278、3936 和 4062 个线性 B 细胞表位的特征描述，对来自尼日利亚的 T. vivax Y486 的基因组进行了 4684 个线性 B 细胞表位的特征描述。所呈现的结果是一个有价值的理论来源，可能为高度敏感和特异性的诊断工具铺平道路。
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