Zoology Department, Faculty of Science, South Valley University, 83523-Qena, Egypt.
Department of Anatomy and Histology, Assiut University, Faculty of Veterinary Medicine, 71515-Assiut, Egypt.
Sci Total Environ. 2018 Jul 15;630:750-756. doi: 10.1016/j.scitotenv.2018.02.205. Epub 2018 Feb 27.
Currently, cadmium is considered to be one of the major environmental pollutants. Environmentally, cadmium is released in various forms e.g. oxide, chloride and sulphide. The aim of the present study was to examine the genotoxic impact of fullerene nanoparticles C (C) and virgin olive oil (VOO) on cadmium chloride (CdCl)-induced genotoxicity in rats. To evaluate these effects on DNA damage and chromosomal frequency, 25 albino rats were randomly assigned to 5 groups (n=5 per group): Group 1 served as a control; Group 2 received a single intraperitoneal dose of CdCl (3.5mg/kg); Group 3 animals were treated with C (4mg/kg, orally) every other day for 20days; Group 4 received a single intraperitoneal dose of CdCl (3.5mg/kg) and an oral dose of C (4mg/kg); and Group 5 received a single intraperitoneal dose of CdCl (3.5mg/kg) and oral doses of VOO every other day for 20 consecutive days. Genotoxic and anti-genotoxic effects of C and VOO were evaluated in the liver, kidney and bone marrow using molecular and cytogenetic assays. As expected, CdCl and C administration was associated with band number alterations in both liver and kidney; however, C pretreatment recovered to approximately basal number. Surprisingly, C and VOO significantly attenuated the genotoxic effects caused by CdCl in livers and kidneys. In bone marrow, in addition to a reduction in the chromosomal number, several chromosomal aberrations were caused by CdCl. These chromosomal alterations were also reversed by C and VOO. In conclusion, molecular and cytogenetic studies showed that C and VOO exhibit anti-genotoxic agents against CdCl-induced genotoxicity in rats. Further studies are needed to investigate the optimal conditions for potential biomedical applications of these anti-genotoxic agents.
目前，镉被认为是主要的环境污染物之一。在环境中，镉以各种形式释放，如氧化物、氯化物和硫化物。本研究旨在研究富勒烯纳米粒子 C（C）和初榨橄榄油（VOO）对氯化镉（CdCl）诱导的大鼠遗传毒性的遗传毒性影响。为了评估这些对 DNA 损伤和染色体频率的影响，将 25 只白化大鼠随机分配到 5 组（每组 5 只）：第 1 组作为对照；第 2 组腹腔注射 CdCl（3.5mg/kg）；第 3 组动物每隔一天口服 C（4mg/kg），共 20 天；第 4 组腹腔注射 CdCl（3.5mg/kg），并口服 C（4mg/kg）；第 5 组腹腔注射 CdCl（3.5mg/kg），并每隔一天口服 VOO 连续 20 天。使用分子和细胞遗传学检测方法评估 C 和 VOO 在肝脏、肾脏和骨髓中的遗传毒性和抗遗传毒性作用。如预期的那样，CdCl 和 C 的给药与肝脏和肾脏中的带数改变有关；然而，C 的预处理恢复到大约基础数量。令人惊讶的是，C 和 VOO 显著减轻了 CdCl 对肝脏和肾脏的遗传毒性作用。在骨髓中，除了染色体数量减少外，CdCl 还引起了几种染色体异常。这些染色体改变也被 C 和 VOO 逆转。总之，分子和细胞遗传学研究表明，C 和 VOO 对 CdCl 诱导的大鼠遗传毒性具有抗遗传毒性作用。需要进一步研究以调查这些抗遗传毒性剂在潜在的生物医学应用中的最佳条件。
