Vahidifar Ehsan, Sajjadi Seyed Ebrahim, Etebari Mahmoud
Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Department of Pharmacognosy and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran., I.R. Iran.
Res Pharm Sci. 2022 Oct 29;17(6):657-664. doi: 10.4103/1735-5362.359432. eCollection 2022 Dec.
Osthole, a plant-derived coumarin, has shown numerous pharmacological effects. However, its genoprotective effects against cadmium-induced DNA damage have not been determined yet. Therefore, this project aimed to assess the effectiveness of osthole against genotoxicity caused by cadmium.
For this purpose, human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of osthole (40, 60, 80, and 120 µM) 24 h before cadmium chloride (CdCl) treatment (40 µM), and then DNA damage was evaluated by comet assay. Furthermore, DPPH and free thiol group assays were applied to evaluate reactive oxygen species scavenger and antioxidant capacities of osthole.
FINDINGS / RESULTS: In the present study, all concentrations of osthole significantly decreased CdCl-induced DNA damage. Furthermore, the antioxidant properties of the osthole were confirmed by DPPH and free thiol assays.
Overall, the findings of this project revealed that osthole could ameliorate cadmium-induced genotoxicity probably by its antioxidant activity.
蛇床子素是一种植物来源的香豆素，已显示出多种药理作用。然而，其对镉诱导的DNA损伤的基因保护作用尚未确定。因此，本项目旨在评估蛇床子素对镉引起的遗传毒性的有效性。
为此，在氯化镉（CdCl）处理（40 μM）前24小时，将人脐静脉内皮细胞（HUVECs）与不同浓度的蛇床子素（40、60、80和120 μM）孵育，然后通过彗星试验评估DNA损伤。此外，应用DPPH和游离巯基检测来评估蛇床子素的活性氧清除剂和抗氧化能力。
在本研究中，所有浓度的蛇床子素均显著降低了CdCl诱导的DNA损伤。此外，通过DPPH和游离巯基检测证实了蛇床子素的抗氧化特性。
总体而言，本项目的研究结果表明，蛇床子素可能通过其抗氧化活性改善镉诱导的遗传毒性。
