电子束辐照的聚吡咯修饰的牛血清白蛋白孔：同时测定肾上腺素和 L-酪氨酸。
Electron beam-irradiated polypyrrole decorated with Bovine serum albumin pores: Simultaneous determination of epinephrine and L-tyrosine.
机构信息
Polymer Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630004, Tamilnadu, India.
Polymer Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630004, Tamilnadu, India.
出版信息
Biosens Bioelectron. 2018 Jun 15;108:53-61. doi: 10.1016/j.bios.2018.02.044. Epub 2018 Feb 21.
In current work highly sensitive and stable electrochemical sensor for simultaneous non-enzymatic detection of epinephrine (EP), L-tyrosine (L-Tyr) is constructed based on Electron beam irradiated Polypyrrole (EB-Ppy) nanospheres (Zeta potential 33.69 mV at pH 7) embedded over bovine serum albumin (BSA) (Zeta potential - 11.54 mV at pH 7) porous structure, fabricated by simple chemical routes. The BSA structure has the advantages of large surface area, excellent structure stability, rich pore channels and redox mediator role. The constructed sensor exhibited excellent sensor performances by the combination of protein with NH group and recorded the linear response of EP, L-Tyr individual in the concentration range of 100 nM-1 mM, 100 nM-800 μM, with detection limit 7.1 nM, 8.8 nM (S/N = 3σ/b). The EB-Ppy-BSA/GCE electrochemical sensor manifests intriguing application with good sensitivity, selectivity and reproducibility towards the EP, L-Tyr detection. The practical analytical utility provides great promise by selective measurements in tea, and chicken extract which has a promising future for biological and healthcare applications.
目前，基于电子束辐照聚吡咯纳米球（Zeta 电位为 33.69 mV，在 pH 值为 7 时）嵌入牛血清白蛋白（Zeta 电位为-11.54 mV，在 pH 值为 7 时）多孔结构，构建了一种用于同时非酶检测肾上腺素（EP）和 L-酪氨酸（L-Tyr）的高灵敏度和稳定的电化学传感器。BSA 结构具有比表面积大、结构稳定性好、孔隙通道丰富和氧化还原介体作用等优点。该构建的传感器通过与具有 NH 基团的蛋白质结合，表现出优异的传感器性能，并记录了 EP、L-Tyr 各自在 100 nM-1 mM、100 nM-800 μM 浓度范围内的线性响应，检测限分别为 7.1 nM 和 8.8 nM（S/N=3σ/b）。EB-Ppy-BSA/GCE 电化学传感器对 EP、L-Tyr 的检测具有良好的灵敏度、选择性和重现性，在茶叶和鸡肉提取物中的选择性测量具有实际分析应用的巨大潜力，为生物和医疗保健应用提供了广阔的前景。
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