Shrestha Bishnu Kumar, Ahmad Rafiq, Mousa Hamouda M, Kim In-Gi, Kim Jeong In, Neupane Madhav Prasad, Park Chan Hee, Kim Cheol Sang
Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
J Colloid Interface Sci. 2016 Nov 15;482:39-47. doi: 10.1016/j.jcis.2016.07.067. Epub 2016 Jul 27.
A highly electroactive bio-nanohybrid film of polypyrrole (PPy)-Nafion (Nf)-functionalized multi-walled carbon nanotubes (fMWCNTs) nanocomposite was prepared on the glassy carbon electrode (GCE) by a facile one-step electrochemical polymerization technique followed by chitosan-glucose oxidase (CH-GOx) immobilization on its surface to achieve a high-performance glucose biosensor. The as-fabricated nanohybrid composite provides high surface area for GOx immobilization and thus enhances the enzyme-loading efficiency. The structural characterization revealed that the PPy-Nf-fMWCNTs nanocomposite films were uniformly formed on GCE and after GOx immobilization, the surface porosities of the film were decreased due to enzyme encapsulation inside the bio-nanohybrid composite materials. The electrochemical behavior of the fabricated biosensor was investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and amperometry measurements. The results indicated an excellent catalytic property of bio-nanohybrid film for glucose detection with improved sensitivity of 2860.3μAmM(-1)cm(-2), the linear range up to 4.7mM (R(2)=0.9992), and a low detection limit of 5μM under a signal/noise (S/N) ratio of 3. Furthermore, the resulting biosensor presented reliable selectivity, better long-term stability, good repeatability, reproducibility, and acceptable measurement of glucose concentration in real serum samples. Thus, this fabricated biosensor provides an efficient and highly sensitive platform for glucose sensing and can open up new avenues for clinical applications.
通过简便的一步电化学聚合技术，在玻碳电极（GCE）上制备了聚吡咯（PPy）-Nafion（Nf）功能化多壁碳纳米管（fMWCNTs）纳米复合材料的高电活性生物纳米杂化膜，随后将壳聚糖-葡萄糖氧化酶（CH-GOx）固定在其表面，以制备高性能葡萄糖生物传感器。所制备的纳米杂化复合材料为GOx固定提供了高表面积，从而提高了酶负载效率。结构表征表明，PPy-Nf-fMWCNTs纳米复合膜在GCE上均匀形成，GOx固定后，由于生物纳米杂化复合材料内部的酶包封，膜的表面孔隙率降低。通过循环伏安法（CV）、电化学阻抗谱（EIS）和安培测量法研究了所制备生物传感器的电化学行为。结果表明，生物纳米杂化膜对葡萄糖检测具有优异的催化性能，灵敏度提高到2860.3μAmM(-1)cm(-2)，线性范围高达4.7mM（R(2)=0.9992），在信号/噪声（S/N）比为3的情况下检测限低至5μM。此外，所得生物传感器具有可靠的选择性、更好的长期稳定性、良好的重复性、再现性，并且能够对真实血清样品中的葡萄糖浓度进行可接受的测量。因此，这种制备的生物传感器为葡萄糖传感提供了一个高效且高度灵敏的平台，并可为临床应用开辟新途径。
