评估长链非编码 RNA AC010761.9 在人胃腺癌中的表达及临床价值。
Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma.
机构信息
Department of Experimental Medicine, Fuzong Clinical Medical College, Fujian Medical University, Fuzhou, China.
Department of Experimental Medicine, Fuzhou General Hospital, 156 North Xi-er Huan Road, Fuzhou, Fujian, 350025, China.
出版信息
World J Surg Oncol. 2018 Mar 2;16(1):40. doi: 10.1186/s12957-017-1289-y.
BACKGROUND
The current study determined the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma (GA).
METHODS
Real-time quantitative reverse transcription (qRT)-PCR was used to detect the level of lncRNA expression in 145 GA tissues and three GA cell lines, and the correlation between its level and clinicopathologic characteristics and potential corresponding mRNA of TNF receptor-associated factor 4 gene (TRAF4) was then evaluated.
RESULTS
Elevated lncRNA AC010761.9 was detected in all 6 GA tissues by previous lncRNA expression profile microarray assay. LncRNA AC010761.9 was over-expressed in 99 of 145 GA tissues (68.3%) with an elevated fold change of up to 35.14 compared to matched paracancerous tissues (p < 0.05), and was also over-expressed in the 3 GA cell lines (MGC803, BGC823, and SGC7901) compared to the normal gastric mucosal epithelial cell line (GES-1 cells; p < 0.05) by qRT-PCR. The elevated expression of this lncRNA was related to tumor size (p = 0.028), degree of differentiation (p = 0.047), and serum carbohydrate antigen (CA19-9) and carcinoembryonic antigen (CEA) concentrations (p = 0.026 and p = 0.037, respectively). Multivariate analysis further confirmed that the expression of lncRNA AC010761.9 was related to the degree of tumor differentiation (p = 0.015). Additionally, the expression of lncRNA AC010761.9 had a positive correlation with the mRNA expression of the potentially associated gene (TRAF4) in GA tissues (r = 0.385, p < 0.01).
CONCLUSIONS
LncRNA AC010761.9 may be linked to GA progression and is a potential new biomarker for GA.
背景
本研究旨在检测长链非编码 RNA AC010761.9 在人胃腺癌（GA）中的表达及其临床意义。
方法
采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 145 例 GA 组织和 3 种 GA 细胞系中 lncRNA 的表达水平，分析其表达水平与临床病理特征的相关性及其潜在相关基因 TNF 受体相关因子 4（TRAF4）mRNA 的相关性。
结果
前期 lncRNA 表达谱微阵列分析显示，AC010761.9 在所有 6 例 GA 组织中均有升高。AC010761.9 在 145 例 GA 组织中有 99 例（68.3%）高表达，与配对癌旁组织相比，其 fold change 高达 35.14（p<0.05），且在 3 种 GA 细胞系（MGC803、BGC823 和 SGC7901）中的表达也高于正常胃黏膜上皮细胞系（GES-1 细胞；p<0.05）。AC010761.9 的高表达与肿瘤大小（p=0.028）、分化程度（p=0.047）以及血清肿瘤标志物 CA19-9 和 CEA 浓度（p=0.026 和 p=0.037）相关。多因素分析进一步证实，lncRNA AC010761.9 的表达与肿瘤分化程度相关（p=0.015）。此外，lncRNA AC010761.9 的表达与 GA 组织中潜在相关基因（TRAF4）的 mRNA 表达呈正相关（r=0.385，p<0.01）。
结论
lncRNA AC010761.9 可能与 GA 的发生发展有关，是一种潜在的 GA 新型生物标志物。
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