基于 TCGA 数据库的胃腺癌患者 lncRNA FOXCUT 表达验证及其对细胞生物学功能的影响。

Verification of expressions of lncRNA FOXCUT in gastric adenocarcinoma patients and its effects on cell biological function based on TCGA database.

机构信息

Department of Gastrointestinal Surgery, Zhuji Affiliated Hospital of Wenzhou Medical University, Shaoxing, P.R. China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6139-6147. doi: 10.26355/eurrev_201907_18427.

DOI:10.26355/eurrev_201907_18427

PMID:31364114

Abstract

OBJECTIVE

This study aimed to investigate expressions of lncRNA FOXCUT in gastric adenocarcinoma patients and its effects on the cell biological function.

PATIENTS AND METHODS

Expressions and survival of lncRNA FOXCUT in gastric adenocarcinoma patients (GA) in the Cancer Genome Atlas (TCGA) database were collected. Fifty patients with GA treated in our hospital (patient group) and another 50 contemporaneous normal people (normal group) were collected. Expressions of lncRNA FOXCUT in GES1, SNU-5, HGC-27, SGC-7901, and AGS cells were detected. Also, si-lncRNA FOXCUT and si-NC sequences were transfected to SGC-7901. Si-RNA and si-NC groups were constructed in AGS cells. QRT-PCR was used to detect expressions of lncRNA FOXCUT in samples. MTT, transwell, and flow cytometry were used to detect the proliferation, invasion, and apoptosis of transfected cells. Patients were followed up for 5 years to observe their survival.

RESULTS

Expressions of lncRNA FOXCUT in cancer tissues of GA patients in TCGA database were significantly increased (p<0.001). The survival rate of patients with low expressions of lncRNA FOXCUT was significantly increased (p=0.017, p=0.047). LncRNA FOXCUT is closely related to patients' tumor diameter, lymph node metastasis, TNM staging, and differentiation degree (p<0.05). LncRNA FOXCUT has high clinical value in disease diagnosis. Multivariate Cox regression analysis found that tumor diameter, lymph node metastasis, and lncRNA FOXCUT were independent prognostic factors. Compared with GES1, expressions of lncRNA FOXCUT in GA cells increased significantly (p<0.05), the proliferation and invasion ability of si-RNA group decreased significantly (p<0.05) compared with si-NC group, and the apoptosis rate of si-RNA group was significantly lower than that of si-NC group (p<0.05).

CONCLUSIONS

We showed that the inhibition of the expressions of lncRNA FOXCUT can reduce the proliferation and invasion of GA cells and increase apoptosis, which can be used as a potential therapeutic target for GA.

摘要

目的

本研究旨在探讨 lncRNA FOXCUT 在胃腺癌患者中的表达及其对细胞生物学功能的影响。

患者和方法

收集癌症基因组图谱（TCGA）数据库中胃腺癌（GA）患者的 lncRNA FOXCUT 表达和生存情况。收集我院治疗的 50 例 GA 患者（患者组）和同期 50 例正常人（正常组）。检测 GES1、SNU-5、HGC-27、SGC-7901 和 AGS 细胞中 lncRNA FOXCUT 的表达。转染 SGC-7901 细胞 si-lncRNA FOXCUT 和 si-NC 序列。构建 AGS 细胞的 si-RNA 和 si-NC 组。采用 QRT-PCR 检测样本中 lncRNA FOXCUT 的表达。MTT、Transwell 和流式细胞术检测转染细胞的增殖、侵袭和凋亡。对患者进行 5 年随访，观察其生存情况。

结果

TCGA 数据库中 GA 患者癌组织中 lncRNA FOXCUT 的表达明显增加（p<0.001）。lncRNA FOXCUT 低表达患者的生存率明显升高（p=0.017，p=0.047）。lncRNA FOXCUT 与患者肿瘤直径、淋巴结转移、TNM 分期和分化程度密切相关（p<0.05）。lncRNA FOXCUT 在疾病诊断中具有较高的临床价值。多因素 Cox 回归分析发现，肿瘤直径、淋巴结转移和 lncRNA FOXCUT 是独立的预后因素。与 GES1 相比，GA 细胞中 lncRNA FOXCUT 的表达明显增加（p<0.05），与 si-NC 组相比，si-RNA 组的增殖和侵袭能力明显降低（p<0.05），si-RNA 组的凋亡率明显低于 si-NC 组（p<0.05）。