Waaijer Mariëtte E C, Gunn David A, van Heemst Diana, Slagboom P Eline, Sedivy John M, Dirks Roeland W, Tanke Hans J, Westendorp Rudi G J, Maier Andrea B
Department of Gerontology and Geriatrics, Leiden University Medical Center, 2300 RC Leiden, the Netherlands.
Unilever Discover, Colworth Science Park, Sharnbrook, Bedfordshire MK44 1LQ, UK.
Aging (Albany NY). 2018 Feb 28;10(2):278-289. doi: 10.18632/aging.101389.
Little is known on how well senescence markers and correlate within individual donors. We studied correlations between the same and different markers. Furthermore, we tested correlations between markers with p16INK4a positivity.From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/ without 0.6 µM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/ without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed .More than half of the correlations of the same senescence markers between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers were not significantly correlated intra-individually with p16INK4a positivity. In conclusion, the same and different senescence markers are frequently correlated significantly within and between experiments, but senescence markers are not correlated with p16INK4a positivity .
关于衰老标志物在个体供体中的相关性如何，目前所知甚少。我们研究了相同和不同标志物之间的相关性。此外，我们还测试了与p16INK4a阳性相关的标志物之间的相关性。从100名供体（20 - 91岁）中获取培养的真皮成纤维细胞，评估其活性氧（ROS）、端粒相关灶（TAF）、p16INK4a和衰老相关β - 半乳糖苷酶（SAβ - gal），在有/无0.6 μM鱼藤酮的情况下培养3天（短期）。在40名供体的成纤维细胞中，另外评估了端粒缩短、ROS和SAβ - gal，在有/无20 nM鱼藤酮的情况下培养7周（长期）。在52名供体的皮肤中，评估了p16INK4a阳性真皮细胞的数量。重复实验之间以及短期与长期实验之间，超过一半的相同衰老标志物的相关性是显著的。在短期和长期实验中，不同衰老标志物之间一半的相关性是显著的。不同的衰老标志物在个体内与p16INK4a阳性无显著相关性。总之，相同和不同的衰老标志物在实验内和实验间经常显著相关，但衰老标志物与p16INK4a阳性不相关。
