Department of Analytical Chemistry, Faculty of Marine and Environmental Sciences, University of Cadiz, Instituto de Investigación Marina (INMAR), Campus of Global International Excellence Cei·Mar. Av. República Saharaui, Puerto Real, 11510 Cádiz, Spain.
Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Campus Rio San Pedro, Puerto Real, 11510 Cádiz, Spain.
Talanta. 2018 May 15;182:210-217. doi: 10.1016/j.talanta.2018.01.092. Epub 2018 Feb 2.
A simple and economical spectrofluorimetric alternative for aluminium determination in bivalve mollusks based on the fluorescent blue-green colour complex between Al(III) and salicylaldehyde picolinoylhydrazone (SAPH) has been studied. The factors that are most likely to affect were optimized with a Box-Behnken design. Optimum conditions were: pH 6.6, 0.9 mol L acetic acid/acetate buffer, 3.0 mmol L SAPH, and 50% ethanol. Detection and quantitation limits were found to be 2.7 μg L and 9.1 μg L, respectively. The upper limit of application was assessed through the limit of linearity which was set as 300 μg L. Intra-day repeatability and inter-day repeatability were evaluated showing an excellent precision for the fluorescence method (both < 5%). The method was sensitive enough for the satisfactory determination of aluminium in several bivalve mollusk samples both fresh and canned seafood. The results showed that commercial fresh wild products presented the smallest Al concentration (6-27 mg per 100 g dry weight), while bivalves preserved in cans the concentration was considerably higher (75 mg per 100 g dry weight). Thus, differences between Al concentration related to processing were identified. The study shows a simple, cost-effective and reliable tool for routine aluminium determination in seafood for food quality control.
基于 Al(III)与水杨醛吡啶甲酰腙（SAPH）之间形成的荧光蓝-绿色络合物，研究了一种用于双壳贝类中铝测定的简单、经济的分光荧光替代方法。通过 Box-Behnken 设计对最有可能影响因素进行了优化。最佳条件为：pH 6.6、0.9 mol/L 乙酸/醋酸盐缓冲液、3.0 mmol/L SAPH 和 50%乙醇。检测限和定量限分别为 2.7 μg/L 和 9.1 μg/L。通过将线性范围的上限设定为 300 μg/L，评估了应用的上限。日内重复性和日间重复性评估表明荧光法具有出色的精密度（均 < 5%）。该方法足够灵敏，可满意地测定新鲜和罐装海鲜等几种双壳贝类样品中的铝。结果表明，商业新鲜野生产品的铝浓度最低（每 100 克干重 6-27 毫克），而罐装双壳贝类的浓度要高得多（每 100 克干重 75 毫克）。因此，确定了与加工相关的铝浓度差异。该研究为食品质量控制中海鲜中铝的常规测定提供了一种简单、经济、可靠的工具。
