College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Talanta. 2018 May 15;182:363-370. doi: 10.1016/j.talanta.2018.02.007. Epub 2018 Feb 5.
In this paper, a FRET (Forster resonance energy transfer) based fluorescence method was developed for the quickly detection of ochratoxin A (OTA) in agricultural products (e.g., flour and beer). A highly fluorescent nitrogen doped carbon dots (CD) were served as energy donor, the DNA and MCH (6-mercapto-1-hexanol) modified Ag nanoparticles were served as energy acceptor in the FRET system. OTA can be detected in a concentration range between 10 and 5000 nM, the limit of detection is 8.7 nM. This method has three advantages: (1) an enhanced fluorescent intensity can be acquired by utilizing the nitrogen doped CD synthesized by one-step approach without sophisticated modification of nanoparticles; (2) OTA detection was accomplished quickly (less than 30 min) by using MCH as assistant molecule; (3) an extended OTA detection linear range was acquired, which may facilitate the OTA detection in real agricultural samples, and is helpful for solving food safety problems.
本文开发了一种基于荧光共振能量转移（FRET）的荧光法，用于快速检测农产品（如面粉和啤酒）中的赭曲霉毒素 A（OTA）。高荧光氮掺杂碳点（CD）被用作能量供体，DNA 和 MCH（6-巯基-1-己醇）修饰的 Ag 纳米颗粒在 FRET 系统中作为能量受体。OTA 可以在 10 到 5000 nM 的浓度范围内检测到，检测限为 8.7 nM。该方法有三个优点：（1）通过使用一步法合成的氮掺杂 CD，可以获得增强的荧光强度，而无需对纳米颗粒进行复杂的修饰；（2）通过使用 MCH 作为辅助分子，快速完成 OTA 检测（少于 30 分钟）；（3）获得了扩展的 OTA 检测线性范围，这有利于在实际农业样品中进行 OTA 检测，有助于解决食品安全问题。
