基于 Gd(OH) 纳晶的电化学发光适体传感器用于食品样品中赭曲霉毒素 A 的检测。
Electrochemiluminescence Aptasensor Based on Gd(OH) Nanocrystalline for Ochratoxin A Detection in Food Samples.
机构信息
College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
出版信息
Biosensors (Basel). 2022 Dec 7;12(12):1141. doi: 10.3390/bios12121141.
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH) nanocrystals with KSO as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL to 10 ng mL, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.
在本研究中，首次研究了以 KSO 为阴极共反应物的 Gd(OH)纳米晶体的电化学发光（ECL）性质。基于该材料突出的 ECL 行为和适体技术的优异特异性，成功构建了用于检测赭曲霉毒素 A（OTA）的 ECL 适体传感器。在 OTA 浓度范围为 0.01 pg mL 至 10 ng mL 时，ECL 信号的变化与 OTA 浓度呈高度线性关系，检测限（LOD）为 0.0027 pg mL。最后，该 ECL 适体传感器进一步用于检测实际样品（葡萄和玉米）中的 OTA，获得了令人满意的结果，这表明所建立的方法有望应用于食品检测。
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