Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Talanta. 2018 May 15;182:428-432. doi: 10.1016/j.talanta.2018.01.068. Epub 2018 Jan 31.
The selective and sensitive detection of dopamine (DA) is of great significance for the identification of schizophrenia, Huntington's disease, and Parkinson's disease from the perspective of molecular diagnostics. So far, most of DA fluorescence sensors are based on the electron transfer from the fluorescence nanomaterials to DA-quinone. However, the limited electron transfer ability of the DA-quinone affects the level of detection sensitivity of these sensors. In this work, based on the DA can reduce Ag into AgNPs followed by oxidized to DA-quinone, we developed a novel silicon nanoparticles-based electron transfer fluorescent sensor for the detection of DA. As electron transfer acceptor, the AgNPs and DA-quinone can quench the fluorescence of silicon nanoparticles effectively through the synergistic electron transfer effect. Compared with traditional fluorescence DA sensors, the proposed synergistic electron transfer-based sensor improves the detection sensitivity to a great extent (at least 10-fold improvement). The proposed sensor shows a low detection limit of DA, which is as low as 0.1 nM under the optimal conditions. This sensor has potential applicability for the detection of DA in practical sample. This work has been demonstrated to contribute to a substantial improvement in the sensitivity of the sensors. It also gives new insight into design electron transfer-based sensors.
从分子诊断学的角度来看，选择性和灵敏地检测多巴胺（DA）对于识别精神分裂症、亨廷顿病和帕金森病具有重要意义。到目前为止，大多数 DA 荧光传感器都是基于荧光纳米材料与 DA-醌之间的电子转移。然而，DA-醌的有限电子转移能力影响了这些传感器的检测灵敏度水平。在这项工作中，我们基于 DA 可以将 Ag 还原成 AgNPs，然后被氧化成 DA-醌，开发了一种基于硅纳米粒子的新型电子转移荧光传感器，用于检测 DA。作为电子转移受体，AgNPs 和 DA-醌可以通过协同电子转移效应有效地猝灭硅纳米粒子的荧光。与传统的荧光 DA 传感器相比，所提出的基于协同电子转移的传感器在很大程度上提高了检测灵敏度（至少提高了 10 倍）。在最佳条件下，该传感器对 DA 的检测限低至 0.1 nM。该传感器在实际样品中检测 DA 具有潜在的适用性。这项工作证明了对传感器灵敏度的显著提高。它还为设计基于电子转移的传感器提供了新的思路。
